Simple Plex Human IL-6 Cartridge

Simple Plex Cartridge Kit containing IL-6 for use with Human Plasma/Serum. Includes wash buffer and sample diluent SD13. For use with the Ella automated immunoassay system
Catalog # Availability Size / Price Qty
SPCKB-PS-000190
SPCKA-PS-000190
SPCKC-PS-000190
Human IL-6 Simple Plex Cartridge Kit for use with the Ella Automated Immunoassay System
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Simple Plex Human IL-6 Cartridge Summary

Sample Type & Volume Required Per Well*
Bronchoalveolar Lavage Fluid (25 uL), Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (13 uL)
Sensitivity
0.26 pg/mL
Assay Range
0.4 - 3,850 pg/mL (Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Specificity
Natural and recombinant human IL-6. 
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IL-6 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), bronchoalveolar lavage fluid (BALF), and cerebrospinal fluid (CSF)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean 47.2 1833 41.5 1800
Standard Deviation 1.17 71 3.5 128
CV% 2.4 3.9 8.3 7.1

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cerebrospinal Fluid (n=8) 103 100-105
EDTA Plasma (n=6) 99 88-112
Heparin Plasma (n=6) 85 70-105
Serum (n=6) 88 74-98

Linearity

Samples containing and/or spiked with high concentrations of human IL-6 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

IL-6 Simple Plex Assay Linearity

Data Examples

Human IL-6 Simple Plex Cartridge Kit for use with the Ella Automated Immunoassay System

IL-6 Simple Plex Assay Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.

Long Name:
Interleukin 6
Entrez Gene IDs:
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names:
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

FAQs

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