Simple Plex Mouse IL-6 Cartridge

Name: Simple Plex Mouse IL-6 Cartridge
Brand: R&D Systems
SKU: SPCKB-MP-000466
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-MP-000466

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Product Documents

Simple Plex Mouse IL-6 assay kit for use on Ella automated benchtop ELISA. Contains cartridge, sample diluent SD09, and wash buffer.

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Cell Culture Supernates (25 µL), Mouse Serum (25 µL), Mouse EDTA Plasma (25 µL), Mouse Heparin Plasma (25 µL)

Sensitivity

0.15 pg/mL

Assay Range

0.6-5770 pg/mL (Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma)

Simple Plex Mouse IL-6 Cartridge Summary

Simple Plex assay is for the detection of mouse Interleukin 6 (IL-6) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all IL-6 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse IL-6.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-6 assay.

Inter-Assay Precision (Precision between assays) Replicates of each IL-6 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse IL-6.

Mouse Cell Culture Supernates, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	27	27
Mean (pg/mL)	31.3	1766	33.0	1722
Standard Deviation	2.67	177	2.78	143
CV%	8.5	10.0	8.4	8.3

Recovery for Simple Plex Mouse IL-6 Cartridge

Recovery for the mouse IL-6 assays was evaluated at three different spiked concentrations of IL-6 protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	103	98-107
Heparin Plasma (n=4)	94	91-96

Linearity

Samples containing and/or spiked with high concentrations of mouse IL-6 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Mouse IL-6 Cartridge

Simple Plex Mouse IL-6 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse IL-6 Assay. Assay range is 0.60-5770 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.

R&D Systems, a Bio-Techne brand, offers a comprehensive range of IL-6 Antibody products, IL-6 Proteins and Enzymes, IL-6 ELISAs, IL-6 Luminex Assays and more. Use the filters above to drill down to the IL-6 product that best fits your needs.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)