STAT3 Antibody (BLR098G)

Name: STAT3 Antibody (BLR098G)
Brand: Novus Biologicals
SKU: NBP3-14691
Price: 519 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-14691

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR098G

View all STAT3 Primary Antibodies »

Product Specifications

Immunogen

between 175 and 225

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for STAT3 Antibody (BLR098G)

Immunoprecipitation: STAT3 Antibody (BLR098G) [NBP3-14691]

Immunoprecipitation: STAT3 Antibody [NBP3-14691] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from NCI-H226 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiSTAT3 recombinant monoclonal antibody(A700-098 lot 1) used for IP at 6 ul per reaction. STAT3was also immunoprecipitated by rabbit anti-STAT3antibody. For blotting immunoprecipitatedSTAT3, was used at 1:1000. Chemiluminescencewith an exposure time of 30 seconds.

STAT3 Antibody [NBP3-14691] -

Rabbit anti-STAT3 recombinant monoclonalantibody [BLR098G]. Secondary: HRPconjugated goat anti-rabbit. IgG Substrate:DAB.

STAT3 Antibody [NBP3-14691] -

Whole cell lysate (50 µg) from RKO, NCI-H226, HEK293T,GaMG, LNCaP, HeLa, Ramos, MOLT-4, and Jurkat cellsprepared using NETN lysis buffer. Antibody: Rabbit antiSTAT3 recombinant monoclonal antibody [BLR098G] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds.Lower Panel: Rabbit anti-Actin recombinant monoclonalantibody [BLR057F].

STAT3 Antibody [NBP3-14691] -

Rabbit anti-STAT3 recombinant monoclonalantibody [BLR098G] Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.

STAT3 Antibody [NBP3-14691] -

Rabbit anti-STAT3 recombinant monoclonal, Lung cancer, antibody [BLR098G], Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.

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Applications for STAT3 Antibody (BLR098G)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunohistochemistry

1:100 to 1:500

Immunoprecipitation

6 µl/1 mg lysate

Western Blot

1:1,000

Application Notes

Epitope retrieval with citrate buffer pH6.0 isrecommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with, BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: STAT3

Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3 is activated by phosphorylation on tyrosine 705 which induces dimerization, nuclear translocation, and DNA binding While, Stat3-activated transcription seems to be regulated by serine phosphorylation at serine 727 (4)

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Gene Symbol

STAT3

Additional STAT3 Products

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Product Specific Notices for STAT3 Antibody (BLR098G)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.