STAT3 Antibody (OTI21E7) - Azide and BSA Free

Name: STAT3 Antibody (OTI21E7) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-74378
Price: 599 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-74378

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # OTI21E7

Format

Azide and BSA Free

View all STAT3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 154-175 of human stat3

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

88.08 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAT3 Antibody (OTI21E7) - Azide and BSA Free

Western Blot: STAT3 Antibody (OTI21E7)Azide and BSA Free [NBP2-74378]

Western Blot: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT3(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT3.

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378]

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378] - Staining of paraffin-embedded thyroid using anti-STAT3 mouse monoclonal antibody.

Western Blot: STAT3 Antibody (OTI21E7)Azide and BSA Free [NBP2-74378]

Western Blot: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378] - Analysis of extracts (35ug) from different cell line by using anti-STAT3 monoclonal antibody. (1:500)

Western Blot: STAT3 Antibody (OTI21E7)Azide and BSA Free [NBP2-74378]

Western Blot: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378] - Analysis of extracts (35ug) from different cell line by using anti-STAT3 monoclonal antibody. (1:500)

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378]

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378] - Staining of paraffin-embedded breast using anti-STAT3 mouse monoclonal antibody.

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378]

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378] - Staining of paraffin-embedded Carcinoma of kidney using anti-STAT3 mouse monoclonal antibody.

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378]

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378] - Staining of paraffin-embedded Carcinoma of lung using anti-STAT3 mouse monoclonal antibody.

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378]

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378] - Staining of paraffin-embedded Carcinoma of pancreas using anti-STAT3 mouse monoclonal antibody.

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378]

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378] - Staining of paraffin-embedded colon using anti-STAT3 mouse monoclonal antibody.

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378]

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378] - Staining of paraffin-embedded kidney using anti-STAT3 mouse monoclonal antibody.

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378]

Immunohistochemistry: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378] - Staining of paraffin-embedded lung using anti-STAT3 mouse monoclonal antibody.

Western Blot: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378]

Western Blot: STAT3 Antibody (OTI21E7) - Azide and BSA Free [NBP2-74378] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells and STAT3-Knockout Hela cells were separated by SDS-PAGE and immunoblotted with anti-STAT3 monoclonal antibody. Then the blotted membrane was stripped and re-probed with anti-b-actin antibody as a loading control.(1:500)

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Applications for STAT3 Antibody (OTI21E7) - Azide and BSA Free

Application

Recommended Usage

Immunohistochemistry

1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: STAT3

Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3 is activated by phosphorylation on tyrosine 705 which induces dimerization, nuclear translocation, and DNA binding While, Stat3-activated transcription seems to be regulated by serine phosphorylation at serine 727 (4)

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Gene Symbol

STAT3

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Product Specific Notices for STAT3 Antibody (OTI21E7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.