STAT5b Antibody (2D1) - Azide and BSA Free

Novus Biologicals | Catalog # H00006777-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D1

Format

Azide and BSA Free
View all STAT5b Primary Antibodies »

Product Specifications

Immunogen

STAT5B (NP_036580, 678 a.a. ~ 787 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VYSKYYTPVPCESATAKAVDGYVKPQIKQVVPEFVNASADAGGGSATYMDQAPSPAVCPQAHYNMYPQNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS

Specificity

STAT5B (2D1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for STAT5b Antibody (2D1) - Azide and BSA Free

Western Blot: STAT5b Antibody (2D1) [H00006777-M03]

Western Blot: STAT5b Antibody (2D1) [H00006777-M03]

Western Blot: STAT5b Antibody (2D1) [H00006777-M03] - Analysis of STAT5B expression in HeLa (Cat # L013V1).
Immunocytochemistry/ Immunofluorescence: STAT5b Antibody (2D1) [H00006777-M03]

Immunocytochemistry/ Immunofluorescence: STAT5b Antibody (2D1) [H00006777-M03]

Immunocytochemistry/Immunofluorescence: STAT5b Antibody (2D1) [H00006777-M03] - Analysis of monoclonal antibody to STAT5B on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: STAT5b Antibody (2D1) [H00006777-M03]

Immunohistochemistry-Paraffin: STAT5b Antibody (2D1) [H00006777-M03]

Immunohistochemistry-Paraffin: STAT5b Antibody (2D1) [H00006777-M03] - Analysis of monoclonal antibody to STAT5B on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 1.5 ug/ml
ELISA: STAT5b Antibody (2D1) [H00006777-M03]

ELISA: STAT5b Antibody (2D1) [H00006777-M03]

ELISA: STAT5b Antibody (2D1) [H00006777-M03] - Detection limit for recombinant GST tagged STAT5B is approximately 0.3ng/ml as a capture antibody.

Applications for STAT5b Antibody (2D1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Reviewed Applications

Read 1 review rated 1 using H00006777-M03 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STAT5b

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.

Long Name

Signal Transducer and Activator of Transcription 5b

Alternate Names

signal transducer and activator of transcription 5B, STAT5, transcription factor STAT5B

Entrez Gene IDs

6777 (Human)

Gene Symbol

STAT5B

OMIM

245590 (Human)

UniProt

NP_036580

Additional STAT5b Products

Product Documents for STAT5b Antibody (2D1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for STAT5b Antibody (2D1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for STAT5b Antibody (2D1) - Azide and BSA Free

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  • STAT5b Antibody (2D1)
    Name: Rognvald Blance
    Application: Simple Western
    Sample Tested: K562 human chronic myelogenous leukemia cell line
    Species: Human
    Verified Customer | Posted 02/06/2017
    Stat5b
    Cells used = K562 or K562 + imatinib - lysed in Protein Simple bicine/CHAPS buffer (040-764) loaded at 100 ng/µL; Gradient = 5 – 8 nested, ladder# 3; Primary antibody at 1:50; Secondary antibody = Protein Simple biotinylated anti-mouse (041-127) with Protein Simple streptavidin-HRP (041-106) both at 1:100.
    STAT5b Antibody (2D1) - Azide and BSA Free H00006777-M03

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Protocols

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FAQs for STAT5b Antibody (2D1) - Azide and BSA Free

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  • Q: For antibodies : STAT5b H00006777-M03; STAT 5a NBR2-00622; STAT3 NBP2-22471; STAT 6 H00006778-M01 - do these detect phosphorylated, unphosphorylated or both forms of STATs?

    A: Antibodies H00006777-M03, NBR2-00622,  NBP2-22471,  and H00006778-M01 were raised to recognize total STAT proteins, they do not differentiate between any posttranslational modifications and will recognize both phosphorylated and non-phosphorylated protein. 

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