TLR Screening Antibody Pack

Novus Biologicals | Catalog # NBP2-25083

Novus Biologicals
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Key Product Details

Species

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Product Summary for TLR Screening Antibody Pack

This pack contains 1 vial each of: NB100-56722SS (0.025 mg), NB100-56723SS (0.025 mg), NB100-56563SS (0.025 mg), NB100-56720SS (0.025 mg), NBP2-24729SS (0.025 mg), NBP2-24827SS (0.025 mg), NBP2-24875SS (0.025 mg), NBP2-24906SS (0.025 mg), NBP2-24917SS (0.025 mg).

Product Specifications

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Scientific Data Images for TLR Screening Antibody Pack

Flow Cytometry: TLR Screening Antibody Pack [NBP2-25083]

Flow Cytometry: TLR Screening Antibody Pack [NBP2-25083]

Flow Cytometry: TLR Screening Antibody Pack [NBP2-25083] - Expression of TLRs in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry.HCMLs were stained using an intracellular staining protocol with TLR-specific antibodies followed by relevant secondary fluorescent-conjugated antibodies. Filled histograms (purple) represent the isotype controls and the open histograms (red) indicate specific antibodies. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license. TLR1 Antibody [NB100-56563], TLR2 Antibody (TL2.1) [NB100-56722], TLR3 Antibody [NBP2-24875], TLR7 Antibody [NBP2-24906], TLR8 Antibody [NBP2-24917], TLR9 Antibody [NBP2-24729]
Immunohistochemistry: TLR Screening Antibody Pack [NBP2-25083]

Immunohistochemistry: TLR Screening Antibody Pack [NBP2-25083]

Immunohistochemistry: TLR Screening Antibody Pack [NBP2-25083] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license. TLR7 Antibody [NBP2-24906]
Western Blot: TLR Screening Antibody Pack [NBP2-25083]

Western Blot: TLR Screening Antibody Pack [NBP2-25083]

Western Blot: TLR Screening Antibody Pack [NBP2-25083] - Expression analysis of TLR protein in HMCLs by Western blotting. All cell lines displayed a strong expression of TLR1, TLR3, TLR4, TLR7, TLR9 proteins. Cell lysates were electrophoresed and blotted to PVDF membrane, which was probed with TLR-specific antibodies. To confirm the immunoreactivity of the antibodies, different positive controls were included. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license. TLR1 Antibody [NB100-56563], TLR3 Antibody [NBP2-24875], TLR7 Antibody [NBP2-24906], TLR9 Antibody [NBP2-24729]
Knockout Validated: TLR Screening Antibody Pack [NBP2-25083]

Western Blot: TLR Screening Antibody Pack [NBP2-25083]

Western Blot: TLR Screening Antibody Pack [NBP2-25083] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092748) licensed under a CC-BY license. TLR9 Antibody (26C593.2) [NBP2-24729]
Immunohistochemistry: TLR Screening Antibody Pack [NBP2-25083]

Immunohistochemistry: TLR Screening Antibody Pack [NBP2-25083]

Immunohistochemistry: TLR Screening Antibody Pack [NBP2-25083] - Representative photographs of immunohistochemistry for tubular TLR2 in G) ctrl, H) ctrl + empa, I) diabetic (dm), J) dm + empagliflozin, K) dm + telmisartan groups (Magnification = original X 200) and quantification of tubular TLR2 expression by Image J (L). (Data are expressed as mean +/- SEM with *=P<0.05 vs ctrl, **=P<0.001 vs ctrl and #=P<0.05 vs dm). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0108994) licensed under a CC-BY license. TLR2 Antibody [NB100-56720]
Simple Western: TLR Screening Antibody Pack [NBP2-25083]

Simple Western: TLR Screening Antibody Pack [NBP2-25083]

Simple Western: TLR Screening Antibody Pack [NBP2-25083] - Simple Western lane view shows a specific band for TLR8 in 0.5 mg/ml of Ramos (left) and Human Spleen (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. TLR8 Antibody (44C143) [NBP2-24917]

Formulation, Preparation, and Storage

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR Screening

The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described

Alternate Names

Toll like receptor, Toll-like receptor, Toll-like receptor (TLR)

Additional TLR Screening Products

Product Documents for TLR Screening Antibody Pack

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TLR Screening Antibody Pack

This pack contains 1 vial each of: NB100-56722SS (0.025 mg), NB100-56723SS (0.025 mg), NB100-56563SS (0.025 mg), NB100-56720SS (0.025 mg), NBP2-24729SS (0.025 mg), NBP2-24827SS (0.025 mg), NBP2-24875SS (0.025 mg), NBP2-24906SS (0.025 mg), NBP2-24917SS (0.025 mg).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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Protocols

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