Total BDNF Quantikine ELISA Kit

Detects free and Trk-bound BDNF
  (6 citations)     
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Citations (6)
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  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor EDTA Plasma (10 uL), Heparin Plasma (50 uL), Platelet-poor Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)
  • Sensitivity
    1.35 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)
  • Specificity
    Natural and recombinant free, trk-bound, pro-, and mature BDNF.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC218 , Quantikine Immunoassay Control Set 885 for Total BDNF - Please Inquire
Product Summary
The Quantikine Total BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure BDNF in cell culture supernates, tissue lysates, serum, plasma, urine, and human milk. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural BDNF.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 97.9 311 586 98.8 296 594
Standard Deviation 3.12 7.52 17.5 7.07 12.7 28
CV% 3.2 2.4 3 7.2 4.3 4.7

Recovery

The recovery of Total BDNF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 97-113
Cell Lysis Buffer (n=4) 94 87-100
Human Milk (n=4) 94 87-99
Platelet-poor EDTA Plasma (n=4) 95 76-110
Platelet-poor Heparin Plasma (n=4) 95 75-111
Urine (n=4) 93 75-106
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total BDNF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 BDNF [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BDNF
BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.
  • Long Name:
    Brain-derived Neurotrophic Factor
  • Entrez Gene IDs:
    627 (Human); 12064 (Mouse); 24225 (Rat)
  • Alternate Names:
    Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; neurotrophin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Sample Type
  1. Expression of brain-derived neurotrophic factor in kidneys from normal and cyclosporine-treated rats
    Authors: YS Tao, SG Piao, YS Jin, JZ Jin, HL Zheng, HY Zhao, SW Lim, CW Yang, C Li
    BMC Nephrol, 2018;19(1):63.
    Species: Rat
    Sample Type: Urine
  2. Neuroprotective effects of intrastriatal injection of rapamycin in a mouse model of excitotoxicity induced by quinolinic acid
    Authors: SW Saliba, EL Vieira, RP Santos, E Candelario, BL Fiebich, LB Vieira, AL Teixeira, AC de Oliveir
    J Neuroinflammation, 2017;14(1):25.
    Species: Mouse
    Sample Type: Tissue Homogenates
  3. Pregnancy promotes pituitary tumors by increasing the rate of the cell cycle
    Authors: C Yin, X Qi
    Oncol Lett, 2017;14(4):4873-4877.
    Species: Mouse
    Sample Type: Serum
  4. Delayed Maturation of Fast-Spiking Interneurons is Rectified by Activation of the TrkB Receptor in the Mouse Model of Fragile X Syndrome
    Authors: T Nomura, TF Musial, JJ Marshall, Y Zhu, CL Remmers, J Xu, DA Nicholson, A Contractor
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
  5. Maternal low-protein diet decreases brain-derived neurotrophic factor expression in the brains of the neonatal rat offspring
    Authors: G Marwarha, K Claycombe-, J Schommer, O Ghribi
    J. Nutr. Biochem., 2017;45(0):54-66.
    Species: Rat
    Sample Type: Tissue Homogenates
  6. Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine
    Authors: Xiao-Su Yang
    Biol Open, 2016;0(0):.
    Species: Rat
    Sample Type: Serum

FAQs

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ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Set 885 for Total BDNF

Ctrl QC218
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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Lysis Buffer 17 (1 x 21 mL)

ELISA 895943 5
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