Total BDNF Quantikine ELISA Kit

Detects free and Trk-bound BDNF
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DBNT00
SBNT00
PDBNT00
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Human/Mouse/Rat/Porcine/Canine Total BDNF ELISA Standard Curve
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Product Details
Citations (36)
FAQs
Supplemental Products
Reviews (3)

Total BDNF Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor EDTA Plasma (10 uL), Heparin Plasma (50 uL), Platelet-poor Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)
Sensitivity
1.35 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)
Specificity
Natural and recombinant free, trk-bound, pro-, and mature BDNF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Total BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure BDNF in cell culture supernates, tissue lysates, serum, plasma, urine, and human milk. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural BDNF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 97.9 311 586 98.8 296 594
Standard Deviation 3.12 7.52 17.5 7.07 12.7 28
CV% 3.2 2.4 3 7.2 4.3 4.7

Recovery

The recovery of Total BDNF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 97-113
Cell Lysis Buffer (n=4) 94 87-100
Human Milk (n=4) 94 87-99
Platelet-poor EDTA Plasma (n=4) 95 76-110
Platelet-poor Heparin Plasma (n=4) 95 75-111
Urine (n=4) 93 75-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total BDNF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human/Mouse/Rat/Porcine/Canine Total BDNF ELISA Linearity

Scientific Data

Human/Mouse/Rat/Porcine/Canine Total BDNF ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BDNF

BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.

Long Name:
Brain-derived Neurotrophic Factor
Entrez Gene IDs:
627 (Human); 12064 (Mouse); 24225 (Rat)
Alternate Names:
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Total BDNF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Dihydromyricetin ameliorates social isolation-induced anxiety by modulating mitochondrial function, antioxidant enzymes, and BDNF
    Authors: AJ Al Omran, S Watanabe, EC Hong, SG Skinner, M Zhang, J Zhang, XM Shao, J Liang
    Neurobiology of stress, 2022;21(0):100499.
    Species: Mouse
    Sample Types: Tissue Homogenates
  2. Intravenous transplantation of amnion-derived mesenchymal stem cells promotes functional recovery and alleviates intestinal dysfunction after spinal cord injury
    Authors: S Takamiya, M Kawabori, K Yamazaki, S Yamaguchi, A Tanimori, K Yamamoto, S Ohnishi, T Seki, K Konno, KK Tha, D Hashimoto, M Watanabe, K Houkin, M Fujimura
    PLoS ONE, 2022;17(7):e0270606.
    Species: Rat
    Sample Types: Tissue Homogenates
  3. The Functional Vision Restorative Effect of Crocin via the BDNF-TrkB Pathway: An In Vivo Study
    Authors: JL Wu, SL Yang, YC Ho, CH Chen, BR Tasi, MC Lee, BY Chen
    Nutrients, 2022;14(9):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Thrombin Preconditioning Improves the Therapeutic Efficacy of Mesenchymal Stem Cells in Severe Intraventricular Hemorrhage Induced Neonatal Rats
    Authors: SY Jung, YE Kim, WS Park, SY Ahn, DK Sung, SI Sung, KM Joo, SG Kim, YS Chang
    International Journal of Molecular Sciences, 2022;23(8):.
    Species: Rat
    Sample Types: CSF
  5. Exploring the relationship between metal exposure, BDNF, and behavior in adolescent males
    Authors: A Rodríguez-, V Mustieles, SC D'Cruz, L Legoff, F Gil, P Olmedo, I Reina-Pére, A Mundo, M Molina, F Smagulova, A David, C Freire, MF Fernández
    Oncogene, 2021;239(0):113877.
    Species: Human
    Sample Types: Serum
  6. An Antagonistic Peptide of Gpr1 Ameliorates LPS-Induced Depression through the Hypothalamic-Pituitary-Ovarian Axis
    Authors: R Li, C Ma, Y Xiong, H Zhao, Y Yang, L Xue, B Wang, T Xiao, J Chen, X Lei, B Ma, J Zhang
    Biomolecules, 2021;11(6):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Exercise-induced increases in Anandamide and BDNF during extinction consolidation contribute to reduced threat following reinstatement: Preliminary evidence from a randomized controlled trial
    Authors: KM Crombie, A Sartin-Tar, K Sellnow, R Ahrenholtz, S Lee, M Matalamaki, NE Almassi, CJ Hillard, KF Koltyn, TG Adams, JM Cisler
    Psychoneuroendocrinology, 2021;132(0):105355.
    Species: Human
    Sample Types: Serum
  8. Dental pulp stem cells stimulate neuronal differentiation of PC12 cells
    Authors: N Sultan, LE Amin, AR Zaher, ME Grawish, BA Scheven
    Neural regeneration research, 2021;16(9):1821-1828.
    Species: Rat
    Sample Types: Cell Culture Supernates
  9. Quercetin protects against diabetic retinopathy in rats by inducing heme oxygenase-1 expression
    Authors: GR Chai, S Liu, HW Yang, XL Chen
    Neural regeneration research, 2021;16(7):1344-1350.
    Species: Rat
    Sample Types: Cell Culture Supernates
  10. Electroacupuncture Ameliorates Cerebral I/R-Induced Inflammation through DOR-BDNF/TrkB Pathway
    Authors: Y Geng, Y Chen, W Sun, Y Gu, Y Zhang, M Li, J Xie, X Tian
    Evid Based Complement Alternat Med, 2020;2020(0):3495836.
    Species: Rat
    Sample Types: Cell Culture Supernates
  11. Computational Analysis of Clinical and Molecular Markers and New Theranostic Possibilities in Primary Open-Angle Glaucoma
    Authors: MD Pinazo-Dur, JJ García-Med, JM Bolarín, SM Sanz-Gonzá, M Valero-Vel, J Abellán-Ab, V Zanón-More, J Moreno-Mon
    Journal of Clinical Medicine, 2020;9(9):.
    Species: Human
    Sample Types: Aqueous Humor
  12. Mindfulness-Based Program Plus Amygdala and Insula Retraining (MAIR) for the Treatment of Women with Fibromyalgia: A Pilot Randomized Controlled Trial
    Authors: JP Sanabria-M, J Montero-Ma, A Feliu-Sole, V Gasión, M Navarro-Gi, H Morillo-Sa, A Colomer-Ca, X Borràs, M Tops, JV Luciano, J García-Cam
    J Clin Med, 2020;9(10):.
    Species: Human
    Sample Types: Serum
  13. RAM-589.555 favors neuroprotective and anti-inflammatory profile of CNS-resident glial cells in acute relapse EAE affected mice
    Authors: R Zilkha-Fal, T Rachutin-Z, L Cleaver, M Gurevich, A Achiron
    J Neuroinflammation, 2020;17(1):313.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Effect of football activity and physical fitness on information processing, inhibitory control and working memory in adolescents
    Authors: RA Williams, SB Cooper, KJ Dring, L Hatch, JG Morris, C Sunderland, ME Nevill
    BMC Public Health, 2020;20(1):1398.
    Species: Human
    Sample Types: Serum
  15. Muscle Injury Induces Postoperative Cognitive Dysfunction
    Authors: L Guéniot, V Lepere, GF De Medeiro, A Danckaert, P Flamant, M Le Dudal, O Langeron, PL Goossens, F Chrétien, G Jouvion
    Sci Rep, 2020;10(1):2768.
    Species: Mouse
    Sample Types: Tissue Homogenates
  16. Alpha-Lipoic Acid Ameliorates Radiation-Induced Salivary Gland Injury by Preserving Parasympathetic Innervation in Rats
    Authors: JH Kim, BK Jeong, SJ Jang, JW Yun, MH Jung, KM Kang, TG Kim, SH Woo
    Int J Mol Sci, 2020;21(7):.
    Species: Rat
    Sample Types: Serum
  17. Long-term effects of experimental carotid stenosis on hippocampal infarct pathology, neurons and glia and amelioration by environmental enrichment
    Authors: W Stevenson, Y Hase, E Wilson, A Hollins, M Hase, A Ennaceur, L Craggs, M Ihara, K Horsburgh, RN Kalaria
    Brain Res. Bull., 2020;163(0):72-83.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. Infusion of Plasma from Exercised Mice Ameliorates Cognitive Dysfunction by Increasing Hippocampal Neuroplasticity and Mitochondrial Functions in 3xTg-AD Mice
    Authors: TW Kim, SS Park, JY Park, HS Park
    Int J Mol Sci, 2020;21(9):.
    Species: Mouse
    Sample Types: Plasma
  19. Systematic Balance Exercises Influence Cortical Activation and Serum BDNF Levels in Older Adults
    Authors: J Kubica, J Szymura, A Domagalik, S Golda, M Wiecek, M Fafrowicz, T Marek, J Pera
    J Clin Med, 2019;8(11):.
    Species: Human
    Sample Types: Serum
  20. Evaluation of Neurosecretome from Mesenchymal Stem Cells Encapsulated in Silk Fibroin Hydrogels
    Authors: Y Martín-Mar, L Fernández-, MH Sanchez-Re, N Marí-Buyé, FJ Rojo, J Pérez-Rigu, M Ramos, GV Guinea, F Panetsos, D González-N
    Sci Rep, 2019;9(1):8801.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Peripheral blood brain-derived neurotrophic factor level and tyrosine kinase B expression on T lymphocytes in systemic lupus erythematosus: Implications for systemic involvement
    Authors: B Tian, C Yang, J Wang, X Hou, S Zhao, Y Li, P Yang
    Cytokine, 2019;123(0):154764.
    Species: Human
    Sample Types: Serum
  22. Insulin treatment protects the brain against neuroinflammation by reducing cerebral cytokines and modulating mitochondrial function
    Authors: P Bortoluzzi, D Casagrande, N Dos Santos, K Ugioni Sim, C Mendes, ME Anastácio, PC Lock Silve, A Pastoris M
    Brain Res. Bull., 2019;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  23. Expression of brain-derived neurotrophic factor in kidneys from normal and cyclosporine-treated rats
    Authors: YS Tao, SG Piao, YS Jin, JZ Jin, HL Zheng, HY Zhao, SW Lim, CW Yang, C Li
    BMC Nephrol, 2018;19(1):63.
    Species: Rat
    Sample Types: Urine
  24. Association of serum BDNF levels and the BDNF Val66Met polymorphism with the sleep pattern in healthy young adults
    Authors: K Saitoh, R Furihata, Y Kaneko, M Suzuki, S Takahashi, M Uchiyama
    PLoS ONE, 2018;13(6):e0199765.
    Species: Human
    Sample Types: Serum
  25. Cell encapsulation enhances antidepressant effect of the mesenchymal stem cells and counteracts depressive-like behavior of treatment-resistant depressed rats
    Authors: K Kin, T Yasuhara, M Kameda, Y Tomita, M Umakoshi, K Kuwahara, I Kin, N Kidani, J Morimoto, M Okazaki, T Sasaki, N Tajiri, CV Borlongan, I Date
    Mol. Psychiatry, 2018;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  26. Increased Calcium Influx through L-type Calcium Channels in Human and Mouse Neural Progenitors Lacking Fragile X Mental Retardation Protein
    Authors: C Danesi, VS Achuta, P Corcoran, UK Peteri, G Turconi, N Matsui, I Albayrak, V Rezov, A Isaksson, ML Castrén
    Stem Cell Reports, 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  27. Aquarobic exercises improve the serum blood irisin and brain-derived neurotrophic factor levels in elderly women
    Authors: JH Kim, DY Kim
    Exp. Gerontol., 2018;104(0):60-65.
    Species: Human
    Sample Types: Serum
  28. BDNF levels are associated with autistic traits in the general population
    Authors: N Brondino, L Fusar-Poli, M Rocchetti, F Bertoglio, N Bloise, L Visai, P Politi
    Psychoneuroendocrinology, 2018;89(0):131-133.
    Species: Human
    Sample Types: Serum
  29. Neuroprotective effects of intrastriatal injection of rapamycin in a mouse model of excitotoxicity induced by quinolinic acid
    Authors: SW Saliba, EL Vieira, RP Santos, E Candelario, BL Fiebich, LB Vieira, AL Teixeira, AC de Oliveir
    J Neuroinflammation, 2017;14(1):25.
    Species: Mouse
    Sample Types: Tissue Homogenates
  30. Delayed Maturation of Fast-Spiking Interneurons is Rectified by Activation of the TrkB Receptor in the Mouse Model of Fragile X Syndrome
    Authors: T Nomura, TF Musial, JJ Marshall, Y Zhu, CL Remmers, J Xu, DA Nicholson, A Contractor
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  31. Aberrant cognitive phenotypes and altered hippocampal BDNF expression related to epigenetic modifications in mice lacking the post-synaptic scaffolding protein SHANK1: Implications for autism spectrum disorder
    Authors: AÖ Sungur, MCE Jochner, H Harb, A K?l?ç, H Garn, RKW Schwarting, M Wöhr
    Hippocampus, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  32. Elevated glutamate and lactate predict brain death after severe head trauma
    Authors: MA Stefani, R Modkovski, G Hansel, ER Zimmer, A Kopczynski, AP Muller, NR Strogulski, MS Rodolphi, RK Carteri, AP Schmidt, JP Oses, DH Smith, LV Portela
    Ann Clin Transl Neurol, 2017;4(6):392-402.
    Species: Human
    Sample Types: CSF
  33. Pregnancy promotes pituitary tumors by increasing the rate of the cell cycle
    Authors: C Yin, X Qi
    Oncol Lett, 2017;14(4):4873-4877.
    Species: Mouse
    Sample Types: Serum
  34. Maternal low-protein diet decreases brain-derived neurotrophic factor expression in the brains of the neonatal rat offspring
    Authors: G Marwarha, K Claycombe-, J Schommer, O Ghribi
    J. Nutr. Biochem., 2017;45(0):54-66.
    Species: Rat
    Sample Types: Tissue Homogenates
  35. Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine
    Authors: Xiao-Su Yang
    Biol Open, 2016;0(0):.
    Species: Rat
    Sample Types: Serum
  36. Initial Observations of Salivary Brain-Derived Neurotrophic Factor Levels in Rett Syndrome.
    Authors: Byiers B, Barney C, Ehrhardt M, Panoskaltsis-Mortari A, Feyma T, Beisang A, Symons F
    Pediatr Neurol, 0;80(0):88-89.
    Species: Human
    Sample Types: Saliva

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Total BDNF Quantikine ELISA Kit
By Anonymous on 11/29/2021
Sample Tested: human

Total BDNF Quantikine ELISA Kit
By Anonymous on 11/23/2021
Sample Tested: Serum

Total BDNF Quantikine ELISA Kit
By Anonymous on 07/25/2019
Sample Tested: rat cortex,rat hippocampus