hh Total BDNF Quantikine ELISA Kit DBNT00: R&D Systems

Total BDNF Quantikine ELISA Kit

Detects free and Trk-bound BDNF.
(1 citations)   
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor EDTA Plasma (10 uL), Heparin Plasma (50 uL), Platelet-poor Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)
  • Sensitivity
    1.35 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)
  • Specificity
    Natural and recombinant free, trk-bound, pro-, and mature BDNF.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC218, Quantikine Immunoassay Control Set 885 for Total BDNF - Please Inquire
Product Summary
The Quantikine Total BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure BDNF in cell culture supernates, tissue lysates, serum, plasma, urine, and human milk. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural BDNF.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean97.931158698.8296594
Standard Deviation3.127.5217.57.0712.728
CV%3.22.437.24.34.7

Recovery

The recovery of Total BDNF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 97-113
Cell Lysis Buffer (n=4) 94 87-100
Human Milk (n=4) 94 87-99
Platelet-poor EDTA Plasma (n=4) 95 76-110
Platelet-poor Heparin Plasma (n=4) 95 75-111
Urine (n=4) 93 75-106
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total BDNF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Total BDNF Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BDNF
BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.
    • Long Name
      Brain-derived Neurotrophic Factor
    • Entrez Gene IDs
      627 (Human); 12064 (Mouse); 24225 (Rat);
    • Alternate Names
      Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; neurotrophin;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    1 Citations: Showing 1 - 1

    1. Neuroprotective effects of intrastriatal injection of rapamycin in a mouse model of excitotoxicity induced by quinolinic acid
      Authors: SW Saliba, EL Vieira, RP Santos, E Candelario, BL Fiebich, LB Vieira, AL Teixeira, AC de Oliveir
      J Neuroinflammation, 2017;14(1):25.
      Species: Mouse
      Sample Type: Tissue Homogenates
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 885 for Total BDNF

    Ctrl QC218
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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    Lysis Buffer 17 (1 x 21 mL)

    ELISA 895943 4
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