Total BDNF Quantikine ELISA Kit

Detects free and Trk-bound BDNF
Catalog # Availability Size / Price Qty
DBNT00
SBNT00
PDBNT00
Control Products Available
Human/Mouse/Rat/Porcine/Canine Total BDNF ELISA Standard Curve
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Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (1)

Total BDNF Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor EDTA Plasma (10 uL), Heparin Plasma (50 uL), Platelet-poor Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)
Sensitivity
1.35 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)
Specificity
Natural and recombinant free, trk-bound, pro-, and mature BDNF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Total BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure BDNF in cell culture supernates, tissue lysates, serum, plasma, urine, and human milk. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural BDNF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 97.9 311 586 98.8 296 594
Standard Deviation 3.12 7.52 17.5 7.07 12.7 28
CV% 3.2 2.4 3 7.2 4.3 4.7

Recovery

The recovery of Total BDNF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 97-113
Cell Lysis Buffer (n=4) 94 87-100
Human Milk (n=4) 94 87-99
Platelet-poor EDTA Plasma (n=4) 95 76-110
Platelet-poor Heparin Plasma (n=4) 95 75-111
Urine (n=4) 93 75-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total BDNF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human/Mouse/Rat/Porcine/Canine Total BDNF ELISA Linearity

Data Examples

Human/Mouse/Rat/Porcine/Canine Total BDNF ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BDNF

BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.

Long Name:
Brain-derived Neurotrophic Factor
Entrez Gene IDs:
627 (Human); 12064 (Mouse); 24225 (Rat)
Alternate Names:
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; neurotrophin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Total BDNF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Insulin treatment protects the brain against neuroinflammation by reducing cerebral cytokines and modulating mitochondrial function
    Authors: P Bortoluzzi, D Casagrande, N Dos Santos, K Ugioni Sim, C Mendes, ME Anastácio, PC Lock Silve, A Pastoris M
    Brain Res. Bull., 2019;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  2. Cell encapsulation enhances antidepressant effect of the mesenchymal stem cells and counteracts depressive-like behavior of treatment-resistant depressed rats
    Authors: K Kin, T Yasuhara, M Kameda, Y Tomita, M Umakoshi, K Kuwahara, I Kin, N Kidani, J Morimoto, M Okazaki, T Sasaki, N Tajiri, CV Borlongan, I Date
    Mol. Psychiatry, 2018;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  3. Association of serum BDNF levels and the BDNF Val66Met polymorphism with the sleep pattern in healthy young adults
    Authors: K Saitoh, R Furihata, Y Kaneko, M Suzuki, S Takahashi, M Uchiyama
    PLoS ONE, 2018;13(6):e0199765.
    Species: Human
    Sample Types: Serum
  4. Expression of brain-derived neurotrophic factor in kidneys from normal and cyclosporine-treated rats
    Authors: YS Tao, SG Piao, YS Jin, JZ Jin, HL Zheng, HY Zhao, SW Lim, CW Yang, C Li
    BMC Nephrol, 2018;19(1):63.
    Species: Rat
    Sample Types: Urine
  5. Increased Calcium Influx through L-type Calcium Channels in Human and Mouse Neural Progenitors Lacking Fragile X Mental Retardation Protein
    Authors: C Danesi, VS Achuta, P Corcoran, UK Peteri, G Turconi, N Matsui, I Albayrak, V Rezov, A Isaksson, ML Castrén
    Stem Cell Reports, 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  6. Delayed Maturation of Fast-Spiking Interneurons is Rectified by Activation of the TrkB Receptor in the Mouse Model of Fragile X Syndrome
    Authors: T Nomura, TF Musial, JJ Marshall, Y Zhu, CL Remmers, J Xu, DA Nicholson, A Contractor
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Pregnancy promotes pituitary tumors by increasing the rate of the cell cycle
    Authors: C Yin, X Qi
    Oncol Lett, 2017;14(4):4873-4877.
    Species: Mouse
    Sample Types: Serum
  8. Elevated glutamate and lactate predict brain death after severe head trauma
    Authors: MA Stefani, R Modkovski, G Hansel, ER Zimmer, A Kopczynski, AP Muller, NR Strogulski, MS Rodolphi, RK Carteri, AP Schmidt, JP Oses, DH Smith, LV Portela
    Ann Clin Transl Neurol, 2017;4(6):392-402.
    Species: Human
    Sample Types: CSF
  9. Aberrant cognitive phenotypes and altered hippocampal BDNF expression related to epigenetic modifications in mice lacking the post-synaptic scaffolding protein SHANK1: Implications for autism spectrum disorder
    Authors: AÖ Sungur, MCE Jochner, H Harb, A K?l?ç, H Garn, RKW Schwarting, M Wöhr
    Hippocampus, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Maternal low-protein diet decreases brain-derived neurotrophic factor expression in the brains of the neonatal rat offspring
    Authors: G Marwarha, K Claycombe-, J Schommer, O Ghribi
    J. Nutr. Biochem., 2017;45(0):54-66.
    Species: Rat
    Sample Types: Tissue Homogenates
  11. Neuroprotective effects of intrastriatal injection of rapamycin in a mouse model of excitotoxicity induced by quinolinic acid
    Authors: SW Saliba, EL Vieira, RP Santos, E Candelario, BL Fiebich, LB Vieira, AL Teixeira, AC de Oliveir
    J Neuroinflammation, 2017;14(1):25.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine
    Authors: Xiao-Su Yang
    Biol Open, 2016;0(0):.
    Species: Rat
    Sample Types: Serum

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Total BDNF Quantikine ELISA Kit
By Anonymous on 07/25/2019
Sample Tested: rat cortex,rat hippocampus