Total BDNF Quantikine ELISA Kit

DBNT00 Datasheet / COA / SDS

Detects free and Trk-bound BDNF

Catalog #	Availability	Size / Price	Qty
DBNT00
SBNT00
PDBNT00

Control Products Available

QC218: Quantikine Immunoassay Control Set 885 for Total BDNF

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Human/Mouse/Rat/Porcine/Canine Total BDNF Standard Curve

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Product Details

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Summary Precision Recovery Linearity Product Datasheets Preparation and Storage Background Related Research Areas

Total BDNF Quantikine ELISA Kit Summary

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor EDTA Plasma (10 uL), Heparin Plasma (50 uL), Platelet-poor Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)

Sensitivity

1.35 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)

Specificity

Natural and recombinant free, trk-bound, pro-, and mature BDNF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Total BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure BDNF in cell culture supernates, tissue lysates, serum, plasma, urine, and human milk. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural BDNF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	97.9	311	586	98.8	296	594
Standard Deviation	3.12	7.52	17.5	7.07	12.7	28
CV%	3.2	2.4	3	7.2	4.3	4.7

Recovery

The recovery of Total BDNF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	103	97-113
Cell Lysis Buffer (n=4)	94	87-100
Human Milk (n=4)	94	87-99
Platelet-poor EDTA Plasma (n=4)	95	76-110
Platelet-poor Heparin Plasma (n=4)	95	75-111
Urine (n=4)	93	75-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total BDNF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BDNF

BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.

Long Name:

Brain-derived Neurotrophic Factor

Entrez Gene IDs:

627 (Human); 12064 (Mouse); 24225 (Rat)

Alternate Names:

Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; neurotrophin

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.