Total BDNF Quantikine ELISA Kit Summary
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Product Summary
Precision
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 97.9 | 311 | 586 | 98.8 | 296 | 594 |
Standard Deviation | 3.12 | 7.52 | 17.5 | 7.07 | 12.7 | 28 |
CV% | 3.2 | 2.4 | 3 | 7.2 | 4.3 | 4.7 |
Recovery
The recovery of Total BDNF spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 103 | 97-113 |
Cell Lysis Buffer (n=4) | 94 | 87-100 |
Human Milk (n=4) | 94 | 87-99 |
Platelet-poor EDTA Plasma (n=4) | 95 | 76-110 |
Platelet-poor Heparin Plasma (n=4) | 95 | 75-111 |
Urine (n=4) | 93 | 75-106 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: BDNF
BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.
Citations for Total BDNF Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Dihydromyricetin ameliorates social isolation-induced anxiety by modulating mitochondrial function, antioxidant enzymes, and BDNF
Authors: AJ Al Omran, S Watanabe, EC Hong, SG Skinner, M Zhang, J Zhang, XM Shao, J Liang
Neurobiology of stress, 2022;21(0):100499.
Species: Mouse
Sample Types: Tissue Homogenates
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Intravenous transplantation of amnion-derived mesenchymal stem cells promotes functional recovery and alleviates intestinal dysfunction after spinal cord injury
Authors: S Takamiya, M Kawabori, K Yamazaki, S Yamaguchi, A Tanimori, K Yamamoto, S Ohnishi, T Seki, K Konno, KK Tha, D Hashimoto, M Watanabe, K Houkin, M Fujimura
PLoS ONE, 2022;17(7):e0270606.
Species: Rat
Sample Types: Tissue Homogenates
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The Functional Vision Restorative Effect of Crocin via the BDNF-TrkB Pathway: An In Vivo Study
Authors: JL Wu, SL Yang, YC Ho, CH Chen, BR Tasi, MC Lee, BY Chen
Nutrients, 2022;14(9):.
Species: Mouse
Sample Types: Tissue Homogenates
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Thrombin Preconditioning Improves the Therapeutic Efficacy of Mesenchymal Stem Cells in Severe Intraventricular Hemorrhage Induced Neonatal Rats
Authors: SY Jung, YE Kim, WS Park, SY Ahn, DK Sung, SI Sung, KM Joo, SG Kim, YS Chang
International Journal of Molecular Sciences, 2022;23(8):.
Species: Rat
Sample Types: CSF
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Exploring the relationship between metal exposure, BDNF, and behavior in adolescent males
Authors: A Rodríguez-, V Mustieles, SC D'Cruz, L Legoff, F Gil, P Olmedo, I Reina-Pére, A Mundo, M Molina, F Smagulova, A David, C Freire, MF Fernández
Oncogene, 2021;239(0):113877.
Species: Human
Sample Types: Serum
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An Antagonistic Peptide of Gpr1 Ameliorates LPS-Induced Depression through the Hypothalamic-Pituitary-Ovarian Axis
Authors: R Li, C Ma, Y Xiong, H Zhao, Y Yang, L Xue, B Wang, T Xiao, J Chen, X Lei, B Ma, J Zhang
Biomolecules, 2021;11(6):.
Species: Mouse
Sample Types: Tissue Homogenates
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Exercise-induced increases in Anandamide and BDNF during extinction consolidation contribute to reduced threat following reinstatement: Preliminary evidence from a randomized controlled trial
Authors: KM Crombie, A Sartin-Tar, K Sellnow, R Ahrenholtz, S Lee, M Matalamaki, NE Almassi, CJ Hillard, KF Koltyn, TG Adams, JM Cisler
Psychoneuroendocrinology, 2021;132(0):105355.
Species: Human
Sample Types: Serum
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Dental pulp stem cells stimulate neuronal differentiation of PC12 cells
Authors: N Sultan, LE Amin, AR Zaher, ME Grawish, BA Scheven
Neural regeneration research, 2021;16(9):1821-1828.
Species: Rat
Sample Types: Cell Culture Supernates
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Quercetin protects against diabetic retinopathy in rats by inducing heme oxygenase-1 expression
Authors: GR Chai, S Liu, HW Yang, XL Chen
Neural regeneration research, 2021;16(7):1344-1350.
Species: Rat
Sample Types: Cell Culture Supernates
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Electroacupuncture Ameliorates Cerebral I/R-Induced Inflammation through DOR-BDNF/TrkB Pathway
Authors: Y Geng, Y Chen, W Sun, Y Gu, Y Zhang, M Li, J Xie, X Tian
Evid Based Complement Alternat Med, 2020;2020(0):3495836.
Species: Rat
Sample Types: Cell Culture Supernates
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Computational Analysis of Clinical and Molecular Markers and New Theranostic Possibilities in Primary Open-Angle Glaucoma
Authors: MD Pinazo-Dur, JJ García-Med, JM Bolarín, SM Sanz-Gonzá, M Valero-Vel, J Abellán-Ab, V Zanón-More, J Moreno-Mon
Journal of Clinical Medicine, 2020;9(9):.
Species: Human
Sample Types: Aqueous Humor
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Mindfulness-Based Program Plus Amygdala and Insula Retraining (MAIR) for the Treatment of Women with Fibromyalgia: A Pilot Randomized Controlled Trial
Authors: JP Sanabria-M, J Montero-Ma, A Feliu-Sole, V Gasión, M Navarro-Gi, H Morillo-Sa, A Colomer-Ca, X Borràs, M Tops, JV Luciano, J García-Cam
J Clin Med, 2020;9(10):.
Species: Human
Sample Types: Serum
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RAM-589.555 favors neuroprotective and anti-inflammatory profile of CNS-resident glial cells in acute relapse EAE affected mice
Authors: R Zilkha-Fal, T Rachutin-Z, L Cleaver, M Gurevich, A Achiron
J Neuroinflammation, 2020;17(1):313.
Species: Mouse
Sample Types: Cell Culture Supernates
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Effect of football activity and physical fitness on information processing, inhibitory control and working memory in adolescents
Authors: RA Williams, SB Cooper, KJ Dring, L Hatch, JG Morris, C Sunderland, ME Nevill
BMC Public Health, 2020;20(1):1398.
Species: Human
Sample Types: Serum
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Muscle Injury Induces Postoperative Cognitive Dysfunction
Authors: L Guéniot, V Lepere, GF De Medeiro, A Danckaert, P Flamant, M Le Dudal, O Langeron, PL Goossens, F Chrétien, G Jouvion
Sci Rep, 2020;10(1):2768.
Species: Mouse
Sample Types: Tissue Homogenates
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Alpha-Lipoic Acid Ameliorates Radiation-Induced Salivary Gland Injury by Preserving Parasympathetic Innervation in Rats
Authors: JH Kim, BK Jeong, SJ Jang, JW Yun, MH Jung, KM Kang, TG Kim, SH Woo
Int J Mol Sci, 2020;21(7):.
Species: Rat
Sample Types: Serum
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Long-term effects of experimental carotid stenosis on hippocampal infarct pathology, neurons and glia and amelioration by environmental enrichment
Authors: W Stevenson, Y Hase, E Wilson, A Hollins, M Hase, A Ennaceur, L Craggs, M Ihara, K Horsburgh, RN Kalaria
Brain Res. Bull., 2020;163(0):72-83.
Species: Mouse
Sample Types: Tissue Homogenates
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Infusion of Plasma from Exercised Mice Ameliorates Cognitive Dysfunction by Increasing Hippocampal Neuroplasticity and Mitochondrial Functions in 3xTg-AD Mice
Authors: TW Kim, SS Park, JY Park, HS Park
Int J Mol Sci, 2020;21(9):.
Species: Mouse
Sample Types: Plasma
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Systematic Balance Exercises Influence Cortical Activation and Serum BDNF Levels in Older Adults
Authors: J Kubica, J Szymura, A Domagalik, S Golda, M Wiecek, M Fafrowicz, T Marek, J Pera
J Clin Med, 2019;8(11):.
Species: Human
Sample Types: Serum
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Evaluation of Neurosecretome from Mesenchymal Stem Cells Encapsulated in Silk Fibroin Hydrogels
Authors: Y Martín-Mar, L Fernández-, MH Sanchez-Re, N Marí-Buyé, FJ Rojo, J Pérez-Rigu, M Ramos, GV Guinea, F Panetsos, D González-N
Sci Rep, 2019;9(1):8801.
Species: Mouse
Sample Types: Cell Culture Supernates
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Peripheral blood brain-derived neurotrophic factor level and tyrosine kinase B expression on T lymphocytes in systemic lupus erythematosus: Implications for systemic involvement
Authors: B Tian, C Yang, J Wang, X Hou, S Zhao, Y Li, P Yang
Cytokine, 2019;123(0):154764.
Species: Human
Sample Types: Serum
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Insulin treatment protects the brain against neuroinflammation by reducing cerebral cytokines and modulating mitochondrial function
Authors: P Bortoluzzi, D Casagrande, N Dos Santos, K Ugioni Sim, C Mendes, ME Anastácio, PC Lock Silve, A Pastoris M
Brain Res. Bull., 2019;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
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Expression of brain-derived neurotrophic factor in kidneys from normal and cyclosporine-treated rats
Authors: YS Tao, SG Piao, YS Jin, JZ Jin, HL Zheng, HY Zhao, SW Lim, CW Yang, C Li
BMC Nephrol, 2018;19(1):63.
Species: Rat
Sample Types: Urine
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Association of serum BDNF levels and the BDNF Val66Met polymorphism with the sleep pattern in healthy young adults
Authors: K Saitoh, R Furihata, Y Kaneko, M Suzuki, S Takahashi, M Uchiyama
PLoS ONE, 2018;13(6):e0199765.
Species: Human
Sample Types: Serum
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Cell encapsulation enhances antidepressant effect of the mesenchymal stem cells and counteracts depressive-like behavior of treatment-resistant depressed rats
Authors: K Kin, T Yasuhara, M Kameda, Y Tomita, M Umakoshi, K Kuwahara, I Kin, N Kidani, J Morimoto, M Okazaki, T Sasaki, N Tajiri, CV Borlongan, I Date
Mol. Psychiatry, 2018;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
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Increased Calcium Influx through L-type Calcium Channels in Human and Mouse Neural Progenitors Lacking Fragile X Mental Retardation Protein
Authors: C Danesi, VS Achuta, P Corcoran, UK Peteri, G Turconi, N Matsui, I Albayrak, V Rezov, A Isaksson, ML Castrén
Stem Cell Reports, 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
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Aquarobic exercises improve the serum blood irisin and brain-derived neurotrophic factor levels in elderly women
Authors: JH Kim, DY Kim
Exp. Gerontol., 2018;104(0):60-65.
Species: Human
Sample Types: Serum
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BDNF levels are associated with autistic traits in the general population
Authors: N Brondino, L Fusar-Poli, M Rocchetti, F Bertoglio, N Bloise, L Visai, P Politi
Psychoneuroendocrinology, 2018;89(0):131-133.
Species: Human
Sample Types: Serum
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Neuroprotective effects of intrastriatal injection of rapamycin in a mouse model of excitotoxicity induced by quinolinic acid
Authors: SW Saliba, EL Vieira, RP Santos, E Candelario, BL Fiebich, LB Vieira, AL Teixeira, AC de Oliveir
J Neuroinflammation, 2017;14(1):25.
Species: Mouse
Sample Types: Tissue Homogenates
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Delayed Maturation of Fast-Spiking Interneurons is Rectified by Activation of the TrkB Receptor in the Mouse Model of Fragile X Syndrome
Authors: T Nomura, TF Musial, JJ Marshall, Y Zhu, CL Remmers, J Xu, DA Nicholson, A Contractor
J. Neurosci., 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Aberrant cognitive phenotypes and altered hippocampal BDNF expression related to epigenetic modifications in mice lacking the post-synaptic scaffolding protein SHANK1: Implications for autism spectrum disorder
Authors: AÖ Sungur, MCE Jochner, H Harb, A K?l?ç, H Garn, RKW Schwarting, M Wöhr
Hippocampus, 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Elevated glutamate and lactate predict brain death after severe head trauma
Authors: MA Stefani, R Modkovski, G Hansel, ER Zimmer, A Kopczynski, AP Muller, NR Strogulski, MS Rodolphi, RK Carteri, AP Schmidt, JP Oses, DH Smith, LV Portela
Ann Clin Transl Neurol, 2017;4(6):392-402.
Species: Human
Sample Types: CSF
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Pregnancy promotes pituitary tumors by increasing the rate of the cell cycle
Authors: C Yin, X Qi
Oncol Lett, 2017;14(4):4873-4877.
Species: Mouse
Sample Types: Serum
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Maternal low-protein diet decreases brain-derived neurotrophic factor expression in the brains of the neonatal rat offspring
Authors: G Marwarha, K Claycombe-, J Schommer, O Ghribi
J. Nutr. Biochem., 2017;45(0):54-66.
Species: Rat
Sample Types: Tissue Homogenates
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Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine
Authors: Xiao-Su Yang
Biol Open, 2016;0(0):.
Species: Rat
Sample Types: Serum
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Initial Observations of Salivary Brain-Derived Neurotrophic Factor Levels in Rett Syndrome.
Authors: Byiers B, Barney C, Ehrhardt M, Panoskaltsis-Mortari A, Feyma T, Beisang A, Symons F
Pediatr Neurol, 0;80(0):88-89.
Species: Human
Sample Types: Saliva
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