Total BDNF Quantikine ELISA Kit
Total BDNF Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 97.9 | 311 | 586 | 98.8 | 296 | 594 |
| Standard Deviation | 3.12 | 7.52 | 17.5 | 7.07 | 12.7 | 28 |
| CV% | 3.2 | 2.4 | 3 | 7.2 | 4.3 | 4.7 |
Recovery
The recovery of Total BDNF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 97-113 |
| Cell Lysis Buffer (n=4) | 94 | 87-100 |
| Human Milk (n=4) | 94 | 87-99 |
| Platelet-poor EDTA Plasma (n=4) | 95 | 76-110 |
| Platelet-poor Heparin Plasma (n=4) | 95 | 75-111 |
| Urine (n=4) | 93 | 75-106 |
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background: BDNF
BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.
Citations for Total BDNF Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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Cell encapsulation enhances antidepressant effect of the mesenchymal stem cells and counteracts depressive-like behavior of treatment-resistant depressed rats
Authors: K Kin, T Yasuhara, M Kameda, Y Tomita, M Umakoshi, K Kuwahara, I Kin, N Kidani, J Morimoto, M Okazaki, T Sasaki, N Tajiri, CV Borlongan, I Date
Mol. Psychiatry, 2018;0(0):.
Species: Rat
Sample Types: Tissue Lysate
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Association of serum BDNF levels and the BDNF Val66Met polymorphism with the sleep pattern in healthy young adults
Authors: K Saitoh, R Furihata, Y Kaneko, M Suzuki, S Takahashi, M Uchiyama
PLoS ONE, 2018;13(6):e0199765.
Species: Human
Sample Types: Serum
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Expression of brain-derived neurotrophic factor in kidneys from normal and cyclosporine-treated rats
Authors: YS Tao, SG Piao, YS Jin, JZ Jin, HL Zheng, HY Zhao, SW Lim, CW Yang, C Li
BMC Nephrol, 2018;19(1):63.
Species: Rat
Sample Types: Urine
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Increased Calcium Influx through L-type Calcium Channels in Human and Mouse Neural Progenitors Lacking Fragile X Mental Retardation Protein
Authors: C Danesi, VS Achuta, P Corcoran, UK Peteri, G Turconi, N Matsui, I Albayrak, V Rezov, A Isaksson, ML Castrén
Stem Cell Reports, 2018;0(0):.
Species: Human
Sample Types: Cell Lysate
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Delayed Maturation of Fast-Spiking Interneurons is Rectified by Activation of the TrkB Receptor in the Mouse Model of Fragile X Syndrome
Authors: T Nomura, TF Musial, JJ Marshall, Y Zhu, CL Remmers, J Xu, DA Nicholson, A Contractor
J. Neurosci., 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Pregnancy promotes pituitary tumors by increasing the rate of the cell cycle
Authors: C Yin, X Qi
Oncol Lett, 2017;14(4):4873-4877.
Species: Mouse
Sample Types: Serum
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Elevated glutamate and lactate predict brain death after severe head trauma
Authors: MA Stefani, R Modkovski, G Hansel, ER Zimmer, A Kopczynski, AP Muller, NR Strogulski, MS Rodolphi, RK Carteri, AP Schmidt, JP Oses, DH Smith, LV Portela
Ann Clin Transl Neurol, 2017;4(6):392-402.
Species: Human
Sample Types: CSF
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Aberrant cognitive phenotypes and altered hippocampal BDNF expression related to epigenetic modifications in mice lacking the post-synaptic scaffolding protein SHANK1: Implications for autism spectrum disorder
Authors: AÖ Sungur, MCE Jochner, H Harb, A K?l?ç, H Garn, RKW Schwarting, M Wöhr
Hippocampus, 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Maternal low-protein diet decreases brain-derived neurotrophic factor expression in the brains of the neonatal rat offspring
Authors: G Marwarha, K Claycombe-, J Schommer, O Ghribi
J. Nutr. Biochem., 2017;45(0):54-66.
Species: Rat
Sample Types: Tissue Homogenates
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Neuroprotective effects of intrastriatal injection of rapamycin in a mouse model of excitotoxicity induced by quinolinic acid
Authors: SW Saliba, EL Vieira, RP Santos, E Candelario, BL Fiebich, LB Vieira, AL Teixeira, AC de Oliveir
J Neuroinflammation, 2017;14(1):25.
Species: Mouse
Sample Types: Tissue Homogenates
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Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine
Authors: Xiao-Su Yang
Biol Open, 2016;0(0):.
Species: Rat
Sample Types: Serum
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