Total BDNF Quantikine ELISA Kit

Name: Total BDNF Quantikine ELISA Kit
Brand: R&D Systems
SKU: PDBNT00
Rating: 5 (1 reviews)

DBNT00 14 Citations 1 Review Datasheet / COA / SDS

Detects free and Trk-bound BDNF

Catalog #	Availability	Size / Price	Qty
DBNT00
SBNT00
PDBNT00

Control Products Available

QC218: Quantikine Immunoassay Control Set 885 for Total BDNF

Bulk Orders

Human/Mouse/Rat/Porcine/Canine Total BDNF ELISA Standard Curve

2 Images

Product Details

Citations (14)

FAQs

Supplemental Products

Reviews (1)

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Total BDNF Quantikine ELISA Kit Summary

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor EDTA Plasma (10 uL), Heparin Plasma (50 uL), Platelet-poor Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)

Sensitivity

1.35 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)

Specificity

Natural and recombinant free, trk-bound, pro-, and mature BDNF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Total BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure BDNF in cell culture supernates, tissue lysates, serum, plasma, urine, and human milk. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural BDNF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	97.9	311	586	98.8	296	594
Standard Deviation	3.12	7.52	17.5	7.07	12.7	28
CV%	3.2	2.4	3	7.2	4.3	4.7

Recovery

The recovery of Total BDNF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	103	97-113
Cell Lysis Buffer (n=4)	94	87-100
Human Milk (n=4)	94	87-99
Platelet-poor EDTA Plasma (n=4)	95	76-110
Platelet-poor Heparin Plasma (n=4)	95	75-111
Urine (n=4)	93	75-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total BDNF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Data Examples

View Larger

Human/Mouse/Rat/Porcine/Canine Total BDNF ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BDNF

BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.

Long Name:

Brain-derived Neurotrophic Factor

Entrez Gene IDs:

627 (Human); 12064 (Mouse); 24225 (Rat)

Alternate Names:

Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Total BDNF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
Filter your results:

Filter by:

Insulin treatment protects the brain against neuroinflammation by reducing cerebral cytokines and modulating mitochondrial function
Authors: P Bortoluzzi, D Casagrande, N Dos Santos, K Ugioni Sim, C Mendes, ME Anastácio, PC Lock Silve, A Pastoris M
Brain Res. Bull., 2019;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
Cell encapsulation enhances antidepressant effect of the mesenchymal stem cells and counteracts depressive-like behavior of treatment-resistant depressed rats
Authors: K Kin, T Yasuhara, M Kameda, Y Tomita, M Umakoshi, K Kuwahara, I Kin, N Kidani, J Morimoto, M Okazaki, T Sasaki, N Tajiri, CV Borlongan, I Date
Mol. Psychiatry, 2018;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
Association of serum BDNF levels and the BDNF Val66Met polymorphism with the sleep pattern in healthy young adults
Authors: K Saitoh, R Furihata, Y Kaneko, M Suzuki, S Takahashi, M Uchiyama
PLoS ONE, 2018;13(6):e0199765.
Species: Human
Sample Types: Serum
Expression of brain-derived neurotrophic factor in kidneys from normal and cyclosporine-treated rats
Authors: YS Tao, SG Piao, YS Jin, JZ Jin, HL Zheng, HY Zhao, SW Lim, CW Yang, C Li
BMC Nephrol, 2018;19(1):63.
Species: Rat
Sample Types: Urine
Increased Calcium Influx through L-type Calcium Channels in Human and Mouse Neural Progenitors Lacking Fragile X Mental Retardation Protein
Authors: C Danesi, VS Achuta, P Corcoran, UK Peteri, G Turconi, N Matsui, I Albayrak, V Rezov, A Isaksson, ML Castrén
Stem Cell Reports, 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Aquarobic exercises improve the serum blood irisin and brain-derived neurotrophic factor levels in elderly women
Authors: JH Kim, DY Kim
Exp. Gerontol., 2018;104(0):60-65.
Species: Human
Sample Types: Serum
BDNF levels are associated with autistic traits in the general population
Authors: N Brondino, L Fusar-Poli, M Rocchetti, F Bertoglio, N Bloise, L Visai, P Politi
Psychoneuroendocrinology, 2018;89(0):131-133.
Species: Human
Sample Types: Serum
Delayed Maturation of Fast-Spiking Interneurons is Rectified by Activation of the TrkB Receptor in the Mouse Model of Fragile X Syndrome
Authors: T Nomura, TF Musial, JJ Marshall, Y Zhu, CL Remmers, J Xu, DA Nicholson, A Contractor
J. Neurosci., 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Pregnancy promotes pituitary tumors by increasing the rate of the cell cycle
Authors: C Yin, X Qi
Oncol Lett, 2017;14(4):4873-4877.
Species: Mouse
Sample Types: Serum
Elevated glutamate and lactate predict brain death after severe head trauma
Authors: MA Stefani, R Modkovski, G Hansel, ER Zimmer, A Kopczynski, AP Muller, NR Strogulski, MS Rodolphi, RK Carteri, AP Schmidt, JP Oses, DH Smith, LV Portela
Ann Clin Transl Neurol, 2017;4(6):392-402.
Species: Human
Sample Types: CSF

Aberrant cognitive phenotypes and altered hippocampal BDNF expression related to epigenetic modifications in mice lacking the post-synaptic scaffolding protein SHANK1: Implications for autism spectrum disorder
Authors: AÖ Sungur, MCE Jochner, H Harb, A K?l?ç, H Garn, RKW Schwarting, M Wöhr
Hippocampus, 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates

Maternal low-protein diet decreases brain-derived neurotrophic factor expression in the brains of the neonatal rat offspring
Authors: G Marwarha, K Claycombe-, J Schommer, O Ghribi
J. Nutr. Biochem., 2017;45(0):54-66.
Species: Rat
Sample Types: Tissue Homogenates

Neuroprotective effects of intrastriatal injection of rapamycin in a mouse model of excitotoxicity induced by quinolinic acid
Authors: SW Saliba, EL Vieira, RP Santos, E Candelario, BL Fiebich, LB Vieira, AL Teixeira, AC de Oliveir
J Neuroinflammation, 2017;14(1):25.
Species: Mouse
Sample Types: Tissue Homogenates

Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine
Authors: Xiao-Su Yang
Biol Open, 2016;0(0):.
Species: Rat
Sample Types: Serum