Total BDNF Quantikine ELISA Kit
Detects free and Trk-bound BDNF
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Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Total BDNF Quantikine ELISA Kit Summary
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor EDTA Plasma (10 uL), Heparin Plasma (50 uL), Platelet-poor Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)
Sensitivity
1.35 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)
Specificity
Natural and recombinant free, trk-bound, pro-, and mature BDNF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Total BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure BDNF in cell culture supernates, tissue lysates, serum, plasma, urine, and human milk. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural BDNF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 97.9 | 311 | 586 | 98.8 | 296 | 594 |
| Standard Deviation | 3.12 | 7.52 | 17.5 | 7.07 | 12.7 | 28 |
| CV% | 3.2 | 2.4 | 3 | 7.2 | 4.3 | 4.7 |
Recovery
The recovery of Total BDNF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 97-113 |
| Cell Lysis Buffer (n=4) | 94 | 87-100 |
| Human Milk (n=4) | 94 | 87-99 |
| Platelet-poor EDTA Plasma (n=4) | 95 | 76-110 |
| Platelet-poor Heparin Plasma (n=4) | 95 | 75-111 |
| Urine (n=4) | 93 | 75-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total BDNF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Data Examples
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BDNF
BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.
Long Name:
Brain-derived Neurotrophic Factor
Entrez Gene IDs:
627 (Human); 12064 (Mouse); 24225 (Rat)
Alternate Names:
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; neurotrophin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Citations for Total BDNF Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Insulin treatment protects the brain against neuroinflammation by reducing cerebral cytokines and modulating mitochondrial function
Authors: P Bortoluzzi, D Casagrande, N Dos Santos, K Ugioni Sim, C Mendes, ME Anastácio, PC Lock Silve, A Pastoris M
Brain Res. Bull., 2019;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
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Cell encapsulation enhances antidepressant effect of the mesenchymal stem cells and counteracts depressive-like behavior of treatment-resistant depressed rats
Authors: K Kin, T Yasuhara, M Kameda, Y Tomita, M Umakoshi, K Kuwahara, I Kin, N Kidani, J Morimoto, M Okazaki, T Sasaki, N Tajiri, CV Borlongan, I Date
Mol. Psychiatry, 2018;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
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Association of serum BDNF levels and the BDNF Val66Met polymorphism with the sleep pattern in healthy young adults
Authors: K Saitoh, R Furihata, Y Kaneko, M Suzuki, S Takahashi, M Uchiyama
PLoS ONE, 2018;13(6):e0199765.
Species: Human
Sample Types: Serum
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Expression of brain-derived neurotrophic factor in kidneys from normal and cyclosporine-treated rats
Authors: YS Tao, SG Piao, YS Jin, JZ Jin, HL Zheng, HY Zhao, SW Lim, CW Yang, C Li
BMC Nephrol, 2018;19(1):63.
Species: Rat
Sample Types: Urine
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Increased Calcium Influx through L-type Calcium Channels in Human and Mouse Neural Progenitors Lacking Fragile X Mental Retardation Protein
Authors: C Danesi, VS Achuta, P Corcoran, UK Peteri, G Turconi, N Matsui, I Albayrak, V Rezov, A Isaksson, ML Castrén
Stem Cell Reports, 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
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Delayed Maturation of Fast-Spiking Interneurons is Rectified by Activation of the TrkB Receptor in the Mouse Model of Fragile X Syndrome
Authors: T Nomura, TF Musial, JJ Marshall, Y Zhu, CL Remmers, J Xu, DA Nicholson, A Contractor
J. Neurosci., 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Pregnancy promotes pituitary tumors by increasing the rate of the cell cycle
Authors: C Yin, X Qi
Oncol Lett, 2017;14(4):4873-4877.
Species: Mouse
Sample Types: Serum
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Elevated glutamate and lactate predict brain death after severe head trauma
Authors: MA Stefani, R Modkovski, G Hansel, ER Zimmer, A Kopczynski, AP Muller, NR Strogulski, MS Rodolphi, RK Carteri, AP Schmidt, JP Oses, DH Smith, LV Portela
Ann Clin Transl Neurol, 2017;4(6):392-402.
Species: Human
Sample Types: CSF
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Aberrant cognitive phenotypes and altered hippocampal BDNF expression related to epigenetic modifications in mice lacking the post-synaptic scaffolding protein SHANK1: Implications for autism spectrum disorder
Authors: AÖ Sungur, MCE Jochner, H Harb, A K?l?ç, H Garn, RKW Schwarting, M Wöhr
Hippocampus, 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Maternal low-protein diet decreases brain-derived neurotrophic factor expression in the brains of the neonatal rat offspring
Authors: G Marwarha, K Claycombe-, J Schommer, O Ghribi
J. Nutr. Biochem., 2017;45(0):54-66.
Species: Rat
Sample Types: Tissue Homogenates
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Neuroprotective effects of intrastriatal injection of rapamycin in a mouse model of excitotoxicity induced by quinolinic acid
Authors: SW Saliba, EL Vieira, RP Santos, E Candelario, BL Fiebich, LB Vieira, AL Teixeira, AC de Oliveir
J Neuroinflammation, 2017;14(1):25.
Species: Mouse
Sample Types: Tissue Homogenates
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Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine
Authors: Xiao-Su Yang
Biol Open, 2016;0(0):.
Species: Rat
Sample Types: Serum
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Total BDNF Quantikine ELISA Kit
By Anonymous on 07/25/2019
Sample Tested: rat cortex,rat hippocampus
