Key Product Details

Species Reactivity

Viral

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 971314
View all Dengue Virus 4 NS1 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant Dengue Virus 4 NS1 protein
Asp1-Ala352
Accession # NP_740318

Specificity

Detects recombinant Dengue Virus 4 NS1 in direct and capture ELISAs and Western blots. In direct ELISAs and Western Blots, no cross-reactivity with recombinant Dengue Virus 1, 2 and 3 NS1 proteins was observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Viral Dengue Virus 4 NS1 Antibody

Detection of Dengue Virus 4 NS1 antibody by Western Blot.

Detection of Dengue Virus 4 NS1 by Western Blot.

Western blot shows recombinant Dengue Virus (DENV) 1, recombinant DENV2, recombinant DENV3, and recombinant DENV4. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Dengue Virus 4 NS1 Monoclonal Antibody (Catalog # MAB9444) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Dengue Virus 4 NS1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Viral Dengue Virus 4 NS1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Dengue Virus 4 NS1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dengue Virus 4 NS1

Dengue virus (DENV), the cause of Dengue fever, is a single positive-stranded RNA virus of the family Flaviviridae, genus Flavivirus. 5 closely related but antigenically distinct serotypes of the virus have been found: DENV-1, DENV-2, DENV-3, DENV-4 and DENV-5.  DENV has a genome of about 11,000 bases, encoding a single large polyprotein that is subsequently cleaved into several structural and non-structural peptides. The polyprotein is divided into three structural proteins, C, prM, E, seven non-structural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5, and short non-coding regions on both the 5' and 3' ends. DENV nonstructural protein-1 (NS1) is a secreted glycoprotein that is absent from viral particles but accumulates in the supernatant and on the plasma membrane of cells during infection.

Long Name

Dengue Virus 4 Dominica/814669/1981 Polyprotein

Alternate Names

DEN-4, Dengue virus Serotype 4, Dengue virus Type 4, DENV4, DENV4-NS1

Entrez Gene IDs

5075729 (Viral)

Gene Symbol

POLY

UniProt

NP_740318

Additional Dengue Virus 4 NS1 Products

Product Documents for Viral Dengue Virus 4 NS1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Viral Dengue Virus 4 NS1 Antibody

For research use only

Related Research Areas

Infectious Diseases

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