Quantikine® High Sensitivity Colorimetric Sandwich ELISA Kits

Quantikine High Sensitivity ELISA Kits are complete assays generally used when very low levels of the target protein are expected. These kits utilize distinct protocols to achieve their impressive levels of analyte detection.

Features

• Detect femtogram levels of protein
• Complete ready-to-use kits
• Exhaustively tested for superior quality and reproducibility
• Detailed protocol booklets include inter- and intra-assay precision, recovery, linearity, sensitivity, and sample value data
• Colorimetric detection

With the amplification system shown in Figure 1, an alkaline phosphatase-based amplification leads to the formation of a colored product. Other kits utilize a polymeric streptavidin-HRP conjugate as shown in Figure 2. Please refer to Product Datasheet for kit-specific information.

Fig 1: Alkaline Phosphatase Detection System

View Full Assay Principle

Kits using Alkaline Phosphatase

• Human IL-10 Quantikine HS ELISA Kit
• Human IL-12 p70 Quantikine HS ELISA Kit
• Human IL-4 Quantikine HS ELISA Kit
• Human IL-7 Quantikine HS ELISA Kit
• Human IL-8/CXCL8 Quantikine HS ELISA Kit
• Human FGF basic Quantikine HS ELISA Kit
• Human GM-CSF Quantikine HS ELISA Kit
• Human PlGF Quantikine HS ELISA Kit

Fig 2: Streptavidin HRP polymer Detection System

View Full Assay Principle

Kits using TMB Substrate

• Human CTLA-4 Quantikine HS ELISA Kit
• Human G-CSF Quantikine HS ELISA Kit
• Human Granzyme B Quantikine HS ELISA Kit
• Human IFN-gamma Quantikine HS ELISA Kit
• Human IL-6 Quantikine HS ELISA Kit
• Human IL-17 Quantikine HS ELISA Kit
• Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
• Human IL-2 Quantikine HS ELISA Kit
• Human TNF-alpha Quantikine HS ELISA
• Mouse IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
• Mouse TNF-alpha Quantikine HS ELISA Kit

Kit Contents

Kit contents may vary slightly. Please refer to product-specific inserts for a complete list of contents for each individual assay.

• Pre-coated 96-well Microplate
• Conjugated Detection Antibody
• Calibrated Immunoassay Standard
• Assay Diluent
• Calibrator Diluent(s)
• Wash Buffer
• Substrate and Substrate Diluent or Color Reagent A and B
• Amplifier and Amplifier Diluent or Streptavidin Polymer-HRP and Streptavidin Polymer-HRP diluent
• Stop Solution
• Plate Sealers

High Sensitivity ELISA Kit Application Examples

Cytokine Release Syndrome (CRS) is a well-known consequence of viral infections such as influenza and COVID-19. It is also an adverse response to cell therapy. IFN-γ secretion is an early and prominent feature of CRS that stimulates a myriad of downstream responses. To learn more about how our high sensitivity ELISAs are used in this space read our IFN-gamma in CRS immunoassay application note.