Anti- Gemtuzumab (Anti-Idiotype) Antibody

Catalog # Availability Size / Price Qty
MAB96171-100
MAB96171-SP
Detection of Gemtuzumab by Western Blot.
5 Images
Product Details
FAQs
Supplemental Products
Reviews

Anti- Gemtuzumab (Anti-Idiotype) Antibody Summary

Specificity
Detects Gemtuzumab in Western blots and direct ELISAs. No cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Trastuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Source
Monoclonal Mouse IgG2A Clone # 975155
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Full length Gemtuzumab
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
ELISA
Detects Gemtuzumab in direct ELISAs.
 
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Gemtuzumab antibody by Western Blot. View Larger

Detection of Gemtuzumab by Western Blot. Western blot shows Rituximab,Trastuzumab, Gemtuzumab, and Brentuximab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti-Gemtuzumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB96171) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Gemtuzumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of anti-Siglec-3/CD33 (Gemtuzumab) on U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of anti-Siglec-3/CD33 (Gemtuzumab) on U937 Human Cell Line by Flow Cytometry. U937 Human histiocytic lymphoma cell line was stained with anti-Siglec-3/CD33 (Gemtuzumab) (Catalog # MAB9635), then Anti-Gemtuzumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB96171, filled histogram) or Mouse IgG2AIsotype Control Antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Specificity of Anti-Gemtuzumab (Anti-Idiotype) Antibody in ELISA Capture Assay. Biotinylated biosimilars were serially diluted and incubated with Anti-Gemtuzumab (Anti-Idiotype) Antibody (Catalog # MAB96171, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Rituximab (black line) or biotinylated IgG control (orange line) was observed.

Specificity of Anti-Gemtuzumab (Anti-Idiotype) Antibody in Direct ELISA. A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Gemtuzumab (Anti-Idiotype) Antibody (Catalog # MAB96171, 333 ng/mL) detected Gemtuzumab. No cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Trastuzumab, Solanezumab, Teprotumumab, and Aducanumab was observed.

Anti-Gemtuzumab Bridging Assay. The anti-Idiotype antibody (catalog number MAB96171) directed against Gemtuzumab was coated at 4 μg/mL in a 96-well polystyrene plate to capture the biosimilar Gemtuzumab. Biotinylated Anti-Gemtuzumab used at 0.5 μg/mLwas used as the detection antibody before incubation with Streptavidin-HRP. Other biosimilars did not bridge the anti-idotype antibody, showing specificity of the anti-Gemtuzumab (anti-Idiotype) antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gemtuzumab

The anti-Gemtuzumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Gemtuzumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Alternate Names
Gemtuzumab

Product Datasheets

FAQs

  1. What is an anti-idiotype antibody?

    • An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Anti- Gemtuzumab (Anti-Idiotype) Antibody

There are currently no reviews for this product. Be the first to review Anti- Gemtuzumab (Anti-Idiotype) Antibody and earn rewards!

Have you used Anti- Gemtuzumab (Anti-Idiotype) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review