B-Raf Antibody (1H12)

Name: B-Raf Antibody (1H12)
Brand: Novus Biologicals
SKU: NBP1-47433
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-47433

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 1H12

View all B-Raf Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human B-Raf expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for B-Raf Antibody (1H12)

Western Blot: B-Raf Antibody (1H12)BSA Free [NBP1-47433]

Western Blot: B-Raf Antibody (1H12) [NBP1-47433] - Analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.

Immunohistochemistry-Paraffin: B-Raf Antibody (1H12) - BSA Free [NBP1-47433]

Immunohistochemistry-Paraffin: B-Raf Antibody (1H12) [NBP1-47433] - Analysis of human testis tissues using BRAF mouse mAb.

Immunohistochemistry-Paraffin: B-Raf Antibody (1H12) - BSA Free [NBP1-47433]

Immunohistochemistry-Paraffin: B-Raf Antibody (1H12) [NBP1-47433] - Analysis of MCF-7 (left) and HepG2 (right) cells using BRAF mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye

Applications for B-Raf Antibody (1H12)

Application

Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Entrez Gene IDs

673 (Human); 109880 (Mouse)

Gene Symbol

BRAF

UniProt

P15056

Additional B-Raf Products

Product Documents for B-Raf Antibody (1H12)

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Product Specific Notices for B-Raf Antibody (1H12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.