B-Raf Antibody - BSA Free

Name: B-Raf Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-89942
Price: 589 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-89942

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all B-Raf Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for B-Raf Antibody - BSA Free

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942]

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942]

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942]

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942]

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human kidney shows moderate cytoplasmic positivity in distal tubules.

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942]

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Western Blot: B-Raf Antibody - BSA Free [NBP1-89942]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Western Blot: B-Raf Antibody [NBP1-89942] -

Western Blot: B-Raf Antibody [NBP1-89942] - Highly efficient wild-type down-regulation & concomitant mutant complementation for BRAF, HRAS & SHP2 proteins by TEMTAC.(a) Expression & phenotype analysis of BRAF V600E & WT in HEK293 cells is shown. Left panel: Western blot results using specific antibodies as indicated. Right panel: MEKp quantification after intensity analysis using ImageJ. (b) Expression & phenotype analysis of HRAS G12V & WT in HEK293 cells. Left panel: Western blot results using antibodies as indicated. Right panel: MEKp quantification after intensity analysis using ImageJ. (c) Expression & phenotype analysis of SHP2 C459G, D61G & WT in GH-HEK293 cells (HEK293 cells stably expressing the growth hormone receptor). Left panel: Western blot results using antibodies as indicated. Right panel: ERKp quantification after intensity analysis using ImageJ. The dashed line indicates that the blot has been cropped. The full blot is provided in Supplementary Figure S11. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26612112), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: B-Raf Antibody - BSA Free [NBP1-89942]

Analysis in human cell line MOLT-4.

Simple Western: B-Raf Antibody [NBP1-89942]

Simple Western: B-Raf Antibody [NBP1-89942] - Simple Western lane view shows a specific band for BRAF in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: B-Raf Antibody [NBP1-89942]

Simple Western: B-Raf Antibody [NBP1-89942] - Electropherogram image(s) of corresponding Simple Western lane view. B-Raf antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).

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Applications for B-Raf Antibody - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 96 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

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Product Specific Notices for B-Raf Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.