Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

HRP

Antibody Source

Polyclonal Rabbit IgG
View all B-Raf Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid peptide near the center of human B-raf. The immunogen is located within amino acids 330 - 380 of B-raf. Amino Acid Squence: QPFRPADEDHRNQFGQRDR

Specificity

Human B-raf has one isoform (766aa, 84kD). Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD). Rat B-raf a has one isoform (712aa, 80kD). NBP2-41219 can detect in human B-raf and mouse isoform 1. This antibody will not cross-react with C-raf.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for B-Raf Antibody [HRP]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

Additional B-Raf Products

Product Documents for B-Raf Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for B-Raf Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

Customer Reviews for B-Raf Antibody [HRP]

There are currently no reviews for this product. Be the first to review B-Raf Antibody [HRP] and earn rewards!

Have you used B-Raf Antibody [HRP]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies