Canine IL‑18/IL‑1F4 Antibody

Name: Canine IL‑18/IL‑1F4 Antibody
Brand: R&D Systems
SKU: MAB2924
Price: 409 USD
Availability: InStock

R&D Systems | Catalog # MAB2924

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Canine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 314820

View all IL-18/IL-1F4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant canine IL‑18/IL‑1F4
Tyr37-Ser193
Accession # Q9XSR0

Specificity

Detects canine IL‑18/IL‑1F4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-18, recombinant mouse (rm) IL-18, recombinant porcine IL-18, recombinant rat IL-18, recombinant rhesus macaque IL-18, rhIL‑36Ra, rhIL-1F7, rhIL-36 gamma, rhIL-1F10, rmIL-1ra, rmIL-36 gamma, or rmIL-36 alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Canine IL‑18/IL‑1F4 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Canine IL‑18/IL‑1F4

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-18/IL-1F4

Interleukin 18, also known as interferon-gamma-inducing factor (IGIF) or IL-1 gamma, is an 18 kDa member of the IL-1 superfamily which shares biologic activities with IL‑12. IL-18 is expressed by liver cells, monocyte/macrophages, osteoblasts and keratinocytes. Mature canine IL-18 shares 73% sequence identity with human IL-18. Caspase-1 (IL-1 beta-converting enzyme) has been shown to process pro-IL-18 to active IL-18. Human IL-18 has been shown to enhance NK cell activity, induce the production of IFN-gamma and GM-CSF and inhibit the production of IL-10 by PBMCs.