Key Product Details

Species Reactivity

Cotton Rat

Applications

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 163426
View all CXCL10/IP-10/CRG-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant cotton rat CXCL10/IP‑10/CRG‑2
Ile22-Val94
Accession # AAL16935

Specificity

Detects cotton rat CXCL10/IP‑10/CRG‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CXCL1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, or 16, recombinant mouse CXCL1, 2, 4, 6, 7, 9, 10, 11, 12, 13, 15 or 16, recombinant rat CXCL1, 2, 3, 5 or recombinant porcine CXCL8  is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Cotton Rat CXCL10/IP‑10/CRG‑2 Antibody

Chemotaxis Induced by CXCL10/IP‑10/CRG‑2 and Neutralization by Cotton Rat CXCL10/IP‑10/CRG‑2 Antibody.

Chemotaxis Induced by CXCL10/IP‑10/CRG‑2 and Neutralization by Cotton Rat CXCL10/IP‑10/CRG‑2 Antibody.

Recombinant Cotton Rat CXCL10/IP-10/CRG-2 (Catalog # 1117-IP) chemo-attracts the BaF3 mouse pro-B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CXCL10/IP-10/CRG-2 (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat CXCL10/ IP-10/CRG-2 Monoclonal Antibody (Catalog # MAB1117). The ND50 is typically 20-40 µg/mL.

Applications for Cotton Rat CXCL10/IP‑10/CRG‑2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Cotton Rat CXCL10/IP-10/CRG-2 (Catalog # 1117-IP) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR3. The Neutralization Dose (ND50) is typically 20-40 µg/mL in the presence of 10 µg/mL Recombinant Cotton Rat CXCL10/IP‑10/CRG‑2.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL10/IP-10/CRG-2

IP-10, also named mob-1 in rat and crg-2 in mouse, is a member of the non-ELR-motif containing CXC chemokine subfamily and has been designated CXCL10. It is induced in various cell types (including keratinocytes, endothelial cells, fibroblasts, smooth muscle cells, monocytes/macrophages, astrocytes, neutrophils, hepatocytes, T cells, epithelial cells and mesothelial cells) in response to stimulation by type I and/or type II interferon as well as by LPS. IP-10 has been shown to chemoattract activated CD4+ and CD8+ T cells, activated NK cells and smooth muscle cells. It also induces the adhesion of activated T cells to VCAM and ICAM. In addition, IP-10 has been reported to antagonize the angiogenic activity of FGF. IP-10 binds to and activates CXCR3, the chemokine receptor that is shared by MIG/CXCL9 and I-TAC/CXCL11, two additional non-ELR CXC chemokines that are induced by interferon. Cotton rat IP-10 cDNA encodes a 94 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 73 aa mature protein with one potential N-linked glycosylation site. Mature cotton rat IP-10 shares 66%, 64%, and 66% aa sequence identity with rat, mouse and human IP10, respectively (1‑7).

References

  1. Vanguri, P. and J.M. Farber (1990) J. Biol. Chem. 265:15049.
  2. Liang, P. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:12515.
  3. Proost, P. et al. (1993) J. Immunol. 150:1000.
  4. Blanco, J.C. et al. (2001) GenBank Accession # AAL16959.
  5. Neville, L.F. (1997) Prog. Growth Factor Res. 8:207.
  6. Belperio, J.A. et al. (2000) J. Leukoc. Biol. 68:1.
  7. Soejima, K. and B.J. Rollins (2001) J. Immunol. 167:6576.

Alternate Names

CRG-2, CRG2, IP-10

Entrez Gene IDs

3627 (Human); 15945 (Mouse)

Gene Symbol

CXCL10

UniProt

AAL16935

Additional CXCL10/IP-10/CRG-2 Products

Product Documents for Cotton Rat CXCL10/IP‑10/CRG‑2 Antibody

Certificate of Analysis

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Product Specific Notices for Cotton Rat CXCL10/IP‑10/CRG‑2 Antibody

For research use only

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