Key Product Details

Species Reactivity

Cotton Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CXCL10/IP-10/CRG-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant cotton rat CXCL10/IP‑10/CRG‑2
Thr27-Val94
Accession # AAL16935

Specificity

Detects cotton rat CXCL10/IP‑10/CRG‑2  in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CXCL-16 is observed. In Western blots, less than 5% cross‑reactivity with recombinant human CXCL10 and recombinant mouse CRG-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Cotton Rat CXCL10/IP‑10/CRG‑2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Cotton Rat CXCL10/IP‑10/CRG‑2 (Catalog # 1117-IP)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL10/IP-10/CRG-2

IP-10, also named mob-1 in rat and crg-2 in mouse, is a member of the non-ELR-motif containing CXC chemokine subfamily and has been designated CXCL10. It is induced in various cell types (including keratinocytes, endothelial cells, fibroblasts, smooth muscle cells, monocytes/macrophages, astrocytes, neutrophils, hepatocytes, T cells, epithelial cells and mesothelial cells) in response to stimulation by type I and/or type II interferon as well as by LPS. IP-10 has been shown to chemoattract activated CD4+ and CD8+ T cells, activated NK cells and smooth muscle cells. It also induces the adhesion of activated T cells to VCAM and ICAM. In addition, IP-10 has been reported to antagonize the angiogenic activity of FGF. IP-10 binds to and activates CXCR3, the chemokine receptor that is shared by MIG/CXCL9 and I-TAC/CXCL11, two additional non-ELR CXC chemokines that are induced by interferon. Cotton rat IP-10 cDNA encodes a 94 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 73 aa mature protein with one potential N-linked glycosylation site. Mature cotton rat IP-10 shares 66%, 64%, and 66% aa sequence identity with rat, mouse and human IP10, respectively (1‑7).

References

  1. Vanguri, P. and J.M. Farber (1990) J. Biol. Chem. 265:15049.
  2. Liang, P. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:12515.
  3. Proost, P. et al. (1993) J. Immunol. 150:1000.
  4. Blanco, J.C. et al. (2001) GenBank Accession # AAL16959.
  5. Neville, L.F. (1997) Prog. Growth Factor Res. 8:207.
  6. Belperio, J.A. et al. (2000) J. Leukoc. Biol. 68:1.
  7. Soejima, K. and B.J. Rollins (2001) J. Immunol. 167:6576.

Alternate Names

CRG-2, CRG2, IP-10

Entrez Gene IDs

3627 (Human); 15945 (Mouse)

Gene Symbol

CXCL10

UniProt

AAL16935

Additional CXCL10/IP-10/CRG-2 Products

Product Documents for Cotton Rat CXCL10/IP‑10/CRG‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Cotton Rat CXCL10/IP‑10/CRG‑2 Antibody

For research use only

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