Cotton Rat IFN‑ alpha Antibody

Name: Cotton Rat IFN‑ alpha Antibody
Brand: R&D Systems
SKU: AF1082
Price: 439 USD
Availability: InStock

R&D Systems | Catalog # AF1082

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
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Key Product Details

Species Reactivity

Validated:

Cotton Rat

Cited:

Cotton Rat

Applications

Validated:

Western Blot, Neutralization

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all IFN-alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant cotton rat IFN-alpha
Cys24-Lys190 (Cys109Tyr)
Accession # AAL18816

Specificity

Detects cotton rat IFN-alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human IFN-beta and recombinant cotton rat IFN-gamma is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Cotton Rat IFN‑ alpha Antibody

IFN‑ alpha Inhibition of EMCV-induced Cytopathy and Neutralization by Cotton Rat IFN‑ alpha Antibody.

Recombinant Cotton Rat IFN-a (Cys109Tyr) (Catalog # 1082-CR) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Cotton Rat IFN-a (Cys109Tyr) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1082). The ND₅₀ is typically 0.015-0.065 µg/mL.

Applications for Cotton Rat IFN‑ alpha Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Cotton Rat IFN-alpha (Cys109Tyr) (Catalog # 1082-CR)

Neutralization

Measured by its ability to neutralize IFN‑ alpha inhibition of EMCV-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND₅₀) is typically 0.015-0.065 µg/mL in the presence of 0.2 ng/mL Recombinant Cotton Rat IFN‑ alpha (Cys109Tyr).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IFN-alpha

Interferon-alpha, also known as leukocyte interferon, comprises a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R1) and a 125 kDa subunit (IFN-alpha R2) that is involved both in ligand-binding and signal transduction. IFN-alpha is expressed primarily by leukocytes upon activation by viruses, bacteria, cytokines and growth factors. IFN-alpha has been shown to have anti-viral and immunomodulatory activities on target cells. It can also inhibit cell proliferation as well as induce apoptosis (1, 2).

Cotton rat IFN-alpha 1 cDNA encodes a 189 amino acid (aa) residue precursor protein with a putative 23 aa signal sequence and a 166 aa mature protein. The protein contains five cysteines, four of which are involved in two intrachain disulfide bonds. Cotton rat IFN-alpha 1 shares 52%, 67%, 68% and 72% aa sequence identity to human, rat, hamster and mouse IFN-alpha 1, respectively.