Cotton Rat IFN-alpha Antibody

Catalog # Availability Size / Price Qty
IFN‑ alpha  Inhibition of EMCV-induced Cytopathy and Neutralization by Cotton Rat IFN‑ alpha  Antibody.
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Product Details
Citations (1)
Supplemental Products

Cotton Rat IFN-alpha Antibody Summary

Species Reactivity
Cotton Rat
Detects cotton rat IFN-alpha  in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human IFN-beta and recombinant cotton rat IFN-gamma is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant cotton rat IFN-alpha
Cys24-Lys190 (Cys109Tyr)
Accession # AAL18816
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Cotton Rat IFN-alpha (Cys109Tyr) (Catalog # 1082-CR)
Measured by its ability to neutralize IFN‑ alpha inhibition of EMCV-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.015-0.065 µg/mL in the presence of 0.2 ng/mL Recombinant Cotton Rat IFN‑ alpha (Cys109Tyr).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IFN‑ alpha  Inhibition of EMCV-induced Cytopathy and Neutralization by Cotton Rat IFN‑ alpha  Antibody. View Larger

IFN‑ alpha Inhibition of EMCV-induced Cytopathy and Neutralization by Cotton Rat IFN‑ alpha Antibody. Recombinant Cotton Rat IFN-a (Cys109Tyr) (Catalog # 1082-CR) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Cotton Rat IFN-a (Cys109Tyr) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1082). The ND50 is typically 0.015-0.065 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha

Interferon-alpha, also known as leukocyte interferon, comprises a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R1) and a 125 kDa subunit (IFN-alpha R2) that is involved both in ligand-binding and signal transduction. IFN-alpha is expressed primarily by leukocytes upon activation by viruses, bacteria, cytokines and growth factors. IFN-alpha has been shown to have anti-viral and immunomodulatory activities on target cells. It can also inhibit cell proliferation as well as induce apoptosis (1, 2).

Cotton rat IFN-alpha 1 cDNA encodes a 189 amino acid (aa) residue precursor protein with a putative 23 aa signal sequence and a 166 aa mature protein. The protein contains five cysteines, four of which are involved in two intrachain disulfide bonds. Cotton rat IFN-alpha 1 shares 52%, 67%, 68% and 72% aa sequence identity to human, rat, hamster and mouse IFN-alpha 1, respectively.

  1. Domanski, P. and O.R. Colamonici (1996) Cytokine Growth Factor Rev. 7:143.
  2. Pestka, S. (2000) Biopolymers 55:254.
Long Name
Interferon alpha
Entrez Gene IDs
3440 (Human)
Alternate Names
IFNA; IFNA2; IFNA2b; IFNalpha; IFN-alpha; IFN-alphaA; INFA2; interferon alpha 2; LeIF D

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Citation for Cotton Rat IFN-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Synergistic induction of interferon alpha through TLR-3 and TLR-9 agonists identifies CD21 as interferon alpha receptor for the B cell response.
    Authors: Kim D, Niewiesk S
    PLoS Pathog, 2013;9(3):e1003233.
    Species: Cotton Rat
    Sample Types: Whole Cells
    Applications: Neutralization


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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