Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Product is stable for a minimum of 3 months from date of shipment when stored at –20°C in a manual defrost freezer. For optimal stability, store at –80°C. Avoid freeze-thaw cycles.
Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 μg/ml gentamicin sulfate.
Sterility Testing: • PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
Background: Basement Membrane Extracts
Basement Membrane Extract (BME) is a soluble form of basement membrane
purified from Engelbreth-Holm-Swarm (EHS) tumor. The extract gels at
37°C to form a reconstituted basement membrane. The major components of
BME include laminin, collagen IV, entactin, and heparin sulfate
proteoglycan. BME can be used in a multiple applications, under a
variety of cell culture conditions, for maintaining growth or promoting
differentiation of primary endothelial, epithelial, smooth muscle and
stem cells. BME can also be utilized in cell attachment, neurite
outgrowth, angiogenesis, in vitro cell invasion and in vivo
Recently we have developed two additional
formulations of Cultrex® BME known as Cultrex® BME Type 2 and Cultrex®
BME Type 3. Cultrex® BME Type 2 provides a proprietary formulation that
is higher in tensile strength when compared to our original BME, while
Cultrex® BME Type 3 is physiologically aligned with the in vivo solid
tumors environment and is recommended for xenografts and other in vivo
There are many applications for Cultrex® BME, which
require different thicknesses and concentrations. In general, BME at a
protein concentration ≥ 10 mg/ml is used for differentiation studies of
primary cells. For applications such as endothelial cell formation of
capillary-like structures (Tube Formation Assay), the differentiation of
rat aorta tissue into capillary-like structures (Aortic Ring Assay),
epithelial organoid formation, or tumor organoid formation, a thick gel
is needed. Some applications, such as propagation of primary cells,
require a thin layer coating and not a thick gel; therefore, the thin
layer method should be used.
Basement Membrane Extracts
How does Cultrex® BME promote cell differentiation?
All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic substrates, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
Have you used Cultrex PathClear Reduced Growth Factor BME, Type 2?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.