Cultrex PathClear Reduced Growth Factor BME, Type 2
Cultrex PathClear Reduced Growth Factor BME, Type 2 Summary
Gelling Assay - BME gels in less than 30 minutes at 37°C, and maintains the gelled form in culture medium for a minimum of 14 days at 37°C.
Murine Engelbreth-Holm-Swarm (EHS) tumor.
Background: Basement Membrane Extracts
Basement Membrane Extract (BME) is a soluble form of basement membrane
purified from Engelbreth-Holm-Swarm (EHS) tumor. The extract gels at
37°C to form a reconstituted basement membrane. The major components of
BME include laminin, collagen IV, entactin, and heparin sulfate
proteoglycan. BME can be used in a multiple applications, under a
variety of cell culture conditions, for maintaining growth or promoting
differentiation of primary endothelial, epithelial, smooth muscle and
stem cells. BME can also be utilized in cell attachment, neurite
outgrowth, angiogenesis, in vitro cell invasion and in vivo
Recently we have developed two additional formulations of Cultrex® BME known as Cultrex® BME Type 2 and Cultrex® BME Type 3. Cultrex® BME Type 2 provides a proprietary formulation that is higher in tensile strength when compared to our original BME, while Cultrex® BME Type 3 is physiologically aligned with the in vivo solid tumors environment and is recommended for xenografts and other in vivo applications.
There are many applications for Cultrex® BME, which require different thicknesses and concentrations. In general, BME at a protein concentration ≥ 10 mg/ml is used for differentiation studies of primary cells. For applications such as endothelial cell formation of capillary-like structures (Tube Formation Assay), the differentiation of rat aorta tissue into capillary-like structures (Aortic Ring Assay), epithelial organoid formation, or tumor organoid formation, a thick gel is needed. Some applications, such as propagation of primary cells, require a thin layer coating and not a thick gel; therefore, the thin layer method should be used.
Citation for Cultrex PathClear Reduced Growth Factor BME, Type 2
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Crosslinked basement membrane-based coatings enhance glucose sensor function and continuous glucose monitoring in vivo.
Authors: Klueh U, Ludzinska I, Czajkowski C, Qiao Y, Kreutzer D
J Biomed Mater Res A, 0;106(1):7-16.
Sample Types: In Vivo
How does Cultrex® BME promote cell differentiation?
All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic substrates, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
Animal-Free Recombinant Proteins
GMP Recombinant Proteins
Tocris Small Molecules
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