Endothelin Pan Specific DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Endothelin ELISA Standard Curve
Preparation and Storage
Endothelin-1 (ET-1) is produced by many tissues including lung, kidney, brain, endocrine glands, and placenta. It is also expressed by several cell types including, mast cells, endothelial cells, epithelial and smooth muscle cells. The most prominent source is vascular endothelium. ET-1 is best known as a potent vasoconstrictor. Elevated blood ET-1 levels are associated with a variety of diseases, possibly as a function of a stress response.
Citations for Endothelin Pan Specific DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Alteration of Endothelin 1, MCP-1 and Chromogranin A in patients with atrial fibrillation undergoing pulmonary vein isolation
Authors: K Lackermair, S Clauss, T Voigt, I Klier, C Summo, B Hildebrand, T Nickel, HL Estner, S Kääb, R Wakili, U Wilbert-La
PLoS ONE, 2017;12(9):e0184337.
Sample Types: Plasma
Peroxisome proliferator-activated receptor-alpha-mediated transcription of miR-199a2 attenuates endothelin-1 expression via hypoxia-inducible factor-1 alpha.
Authors: Li C, Mpollo M, Gonsalves C, Tahara S, Malik P, Kalra V
J Biol Chem, 2014;289(52):36031-47.
Sample Types: Plasma
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