GSK-3 beta [p Ser9] Antibody (23GB1175)

Name: GSK-3 beta [p Ser9] Antibody (23GB1175)
Brand: Novus Biologicals
SKU: NBP3-51991
Price: 529 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-51991

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1175

View all GSK-3 beta Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-GSK-3 beta (Ser9) (Uniprot: P49841)

Modification

p Ser9

Specificity

This antibody detects endogenous levels of GSK3B only when phosphorylated at S9

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GSK-3 beta [p Ser9] Antibody (23GB1175)

Immunocytochemistry/ Immunofluorescence: GSK-3 beta [p Ser9] Antibody (23GB1175)[NBP3-51991] -

Immunocytochemical staining of HepG2 cells with GSK-3 beta antibody (1:1,000). Nuclei were stained blue with DAPI; GSK-3 beta was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.

Western Blot: GSK-3 beta [p Ser9] Antibody (23GB1175)[NBP3-51991] -

Western blotting analysis using GSK-3 beta (Ser9) antibody (1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for GSK-3 beta [p Ser9] Antibody (23GB1175)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GSK-3 beta

Glycogen synthase kinase 3beta (GSK-3 beta) is a Serine/Threonine protein kinas involved in the insulin and wingless pathways (1-2). Originally identified as a regulator of glycogen synthase, it has been shown to regulate a diverse array of cellular functions. GSK-3 beta activity is down regulated by phosphorylation on Serine 9 by PKB, or activated by phosphorylation on Tyrosine 216. Once activated, the protein can phosphorylate p53, c-jun, heat shock factor-1 and cyclin D1 (3-4). It is also known to phosphorylate Tau in Allzheimer's disease. Additionally, increased GSK-3 beta protein levels are found in Alzheimer's disease brains.

Long Name

Glycogen Synthase Kinase 3 beta

Alternate Names

GSK3 beta, GSK3B

Gene Symbol

GSK3B

Additional GSK-3 beta Products

Product Documents for GSK-3 beta [p Ser9] Antibody (23GB1175)

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Certificate of Analysis

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Product Specific Notices for GSK-3 beta [p Ser9] Antibody (23GB1175)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.