Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1051024
View all Adenosine A1R Primary Antibodies »

Product Specifications

Immunogen

Human Adora-1 synthetic peptide corresponding to the 2nd. extra-cellular loop.

Accession # P30542

Specificity

Detects human Adora-1 in direct and cell-based elisas

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Adenosine A1R Antibody

Detection of Adenosine A1R in human liver cancer.

Adenosine A1R was detected in immersion fixed paraffin-embedded sections of liver cancer using Mouse Anti-Human Adenosine A1R Monoclonal Antibody (Catalog # MAB11135) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with Hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Adenosine A1R Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded human liver cancer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Adenosine A1R

Adenosine A1 Receptor (ADORA1, Adenosine A1R) is an adenosine receptor that belongs to the G protein-coupled receptor family (GPCR). There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The Adenosine A1R receptor inhibits adenylyl cyclase, and plays a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication.

Long Name

Adenosine A1 Receptor

Alternate Names

Adenosine A1 R, ADORA1, RDC7

Entrez Gene IDs

134 (Human); 11539 (Mouse); 29290 (Rat)

Gene Symbol

ADORA1

UniProt

P30542 (Human)

Additional Adenosine A1R Products

Product Documents for Human Adenosine A1R Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Adenosine A1R Antibody

For research use only

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Protocols

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