Human Adenosine A2aR Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9497R-100
MAB9497R-SP
Detection of Adenosine A2a R  in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP by Flow  Cytometry.
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Human Adenosine A2aR Antibody Summary

Species Reactivity
Human
Specificity
Detects human Adenosine A2a R in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG2A Clone # 599717R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Adenosine A2a R
Met1-Ser412
Accession # P29274
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Adenosine A2a R  in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP by Flow  Cytometry. View Larger

Detection of Adenosine A2a R in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Adenosine A2a R or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Adora A2 R Monoclonal Antibody (Catalog # MAB9497R) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosine A2aR

ADORA-A2 (Adenosine A2a Receptor) is a widely expressed seven transmembrane G protein-coupled receptor. Activation by adenosine leads to increased intracellular cAMP levels. ADORA-2A mediates many biological functions, including cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. ADORA-A2 has been identified as a target for therapeutic drugs for inflammation, cancer, ischemic reperfusion injury, diabetic nephropathy, infectious diseases and neuronal disorders.

Long Name
Adenosine A2A Receptor
Entrez Gene IDs
135 (Human); 11540 (Mouse); 25369 (Rat)
Alternate Names
A2aR; adenosine A2 receptor; Adenosine A2a R; adenosine A2a receptor; Adenosine A2aR; adenosine receptor A2a; adenosine receptor subtype A2a; ADORA 2; ADORA2; ADORA2A; hA2aR; RDC8

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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