|Detection of Human AFAP by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human AFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4558) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AFAP at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.|
AFAP (actin filament associated protein; also known as AFAP-110) is an adaptor protein that regulates actin filament integrity. It is both multimodular and multimeric, and is known to simultaneously bind actin and cytosolic kinases. As a homomultimer, AFAP binds and separates actin filaments, preventing actin cross-linking. Upon binding to kinases, AFAP is destabilized, promoting actin polymerization. Human AFAP is 730 amino acids (aa) in length, and contains SH3 and SH2 binding motifs (aa 69‑74 and aa 91‑96), two pleckstrin homology domains (aa 153‑248 and aa 347‑450) and a Leu-zipper/actin binding region (aa 511‑637). Over aa 450‑730, human AFAP is 86% identical to mouse and rat AFAP.
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