Detection of Human alpha 1‑Acid Glycoprotein by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human alpha 1‑Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3694) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for alpha 1‑Acid Glycoprotein at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 1-Acid Glycoprotein
alpha 1-acid Glycoprotein, also known as orosomucoid-1, is a positive
acute-phase reactant that is produced by the liver and secreted in
plasma. Plasma alpha 1-acid concentration increases in response to
inflammation and infection. alpha 1-acid glycoprotein belongs to the
lipocalin family and binds numerous basic and neutral lipophilic drugs
and steroid hormones. It is a 183 amino acid protein with 5 N-linked
glycans that comprises 45% of the 43 kDa mass of the protein. The human
and mouse protein share 43% amino acid sequence identity.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.