Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all alpha 1-Acid Glycoprotein Primary Antibodies »

Product Specifications

Immunogen

Human plasma-derived alpha 1-Acid Glycoprotein

Specificity

Detects human alpha 1-Acid Glycoprotein in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mose alpha 1-Acid Glycoprotein is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human alpha 1‑Acid Glycoprotein Antibody

Detection of Human a1‑Acid Glycoprotein antibody by Western Blot.

Detection of Human alpha 1‑Acid Glycoprotein by Western Blot.

Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human a1-Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3694) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for a1-Acid Glycoprotein at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human a1-Acid Glycoprotein antibody by Simple WesternTM.

Detection of Human alpha 1‑Acid Glycoprotein by Simple WesternTM.

Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for a1-Acid Glycoprotein at approximately 79 kDa (as indicated) using 5 µg/mL of Goat Anti-Human a1-Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3694) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human alpha 1‑Acid Glycoprotein Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: Human serum

Western Blot

0.1 µg/mL
Sample: Human plasma

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha 1-Acid Glycoprotein

alpha 1-acid Glycoprotein, also known as orosomucoid-1, is a positive acute-phase reactant that is produced by the liver and secreted in plasma. Plasma alpha 1-acid concentration increases in response to inflammation and infection. alpha 1-acid glycoprotein belongs to the lipocalin family and binds numerous basic and neutral lipophilic drugs and steroid hormones. It is a 183 amino acid protein with 5 N-linked glycans that comprises 45% of the 43 kDa mass of the protein. The human and mouse protein share 43% amino acid sequence identity.

Alternate Names

AGP, AGP1, AGPA, alpha 1Acid Glycoprotein, ORM1, Orosomucoid

Entrez Gene IDs

5004 (Human); 18405 (Mouse); 24614 (Rat)

Gene Symbol

ORM1

Additional alpha 1-Acid Glycoprotein Products

Product Documents for Human alpha 1‑Acid Glycoprotein Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human alpha 1‑Acid Glycoprotein Antibody

For research use only

Citations for Human alpha 1‑Acid Glycoprotein Antibody

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