Human alpha 1-Acid Glycoprotein Antibody

Catalog # Availability Size / Price Qty
AF3694
AF3694-SP
Detection of Human  alpha 1‑Acid Glycoprotein by Western Blot.
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Product Details
Citations (1)
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Human alpha 1-Acid Glycoprotein Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha 1-Acid Glycoprotein in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mose alpha 1-Acid Glycoprotein is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Human plasma-derived alpha 1-Acid Glycoprotein
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human  alpha 1‑Acid Glycoprotein by Western Blot. View Larger

Detection of Human alpha 1‑Acid Glycoprotein by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human alpha 1‑Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3694) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for alpha 1‑Acid Glycoprotein at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection  of Human  alpha 1‑Acid Glycoprotein by Simple  WesternTM. View Larger

Detection of Human alpha 1‑Acid Glycoprotein by Simple WesternTM. Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for alpha 1‑Acid Glycoprotein at approximately 79 kDa (as indicated) using 5 µg/mL of Goat Anti-Human alpha 1‑Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3694) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 1-Acid Glycoprotein

alpha 1-acid Glycoprotein, also known as orosomucoid-1, is a positive acute-phase reactant that is produced by the liver and secreted in plasma. Plasma alpha 1-acid concentration increases in response to inflammation and infection. alpha 1-acid glycoprotein belongs to the lipocalin family and binds numerous basic and neutral lipophilic drugs and steroid hormones. It is a 183 amino acid protein with 5 N-linked glycans that comprises 45% of the 43 kDa mass of the protein. The human and mouse protein share 43% amino acid sequence identity.

Entrez Gene IDs
5004 (Human); 18405 (Mouse); 24614 (Rat)
Alternate Names
AGP 1; AGP; AGP1; AGP1alpha-1-acid glycoprotein 1; AGPA; AGP-A; alpha 1Acid Glycoprotein; alpha 1-Acid Glycoprotein; OMD 1; ORM; ORM1; orosomucoid 1; Orosomucoid; orosomucoid-1

Product Datasheets

Citation for Human alpha 1-Acid Glycoprotein Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Astrocytic orosomucoid-2 modulates microglial activation and neuroinflammation
    Authors: M Jo, JH Kim, G Song, M Seo, E Mi Hwang, K Suk
    J. Neurosci, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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