Detection of Human alpha 1‑Acid Glycoprotein by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human alpha 1‑Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3694) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for alpha 1‑Acid Glycoprotein at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human alpha 1‑Acid Glycoprotein by Simple WesternTM.
Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for alpha 1‑Acid Glycoprotein at approximately 79 kDa (as indicated) using 5 µg/mL of Goat Anti-Human alpha 1‑Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3694) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 1-Acid Glycoprotein
alpha 1-acid Glycoprotein, also known as orosomucoid-1, is a positive
acute-phase reactant that is produced by the liver and secreted in
plasma. Plasma alpha 1-acid concentration increases in response to
inflammation and infection. alpha 1-acid glycoprotein belongs to the
lipocalin family and binds numerous basic and neutral lipophilic drugs
and steroid hormones. It is a 183 amino acid protein with 5 N-linked
glycans that comprises 45% of the 43 kDa mass of the protein. The human
and mouse protein share 43% amino acid sequence identity.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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