Human Aminopeptidase LRAP/ERAP2 Antibody

R&D Systems | Catalog # AF3830

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Aminopeptidase LRAP/ERAP2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase LRAP/ERAP2
Ala56-Thr960
Accession # Q6P179

Specificity

Detects human Aminopeptidase LRAP/ERAP2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Aminopeptidase LRAP/ERAP2 Antibody

Detection of Human Aminopeptidase LRAP/ERAP2 by Western Blot

Detection of Human Aminopeptidase LRAP/ERAP2 by Western Blot

Immunoblot analyses of ERAP2 using LCL protein extract.Two LCLs of each ERAP2 genotype (AA, AB, and BB) were tested for protein using primary antibodies specific to: A, ERAP2 (goat polyclonal); B, ERAP2 (mouse polyclonal); and C, beta -actin (see Materials and Methods). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1001157), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Aminopeptidase LRAP/ERAP2 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase LRAP/ERAP2 (Catalog # 3830-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Aminopeptidase LRAP/ERAP2 (Catalog # 3830-ZN)

Reviewed Applications

Read 1 review rated 4 using AF3830 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Aminopeptidase LRAP/ERAP2

Leukocyte-derived arginine aminopeptidase (LRAP) and endoplasmic reticulum-aminopeptidase 2 (ERAP2) are the two names given to the protein encoded by the LRAP gene (1, 2). Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors. It belongs to the oxytocinase subfamily of M1 aminopeptidases, which also includes aminopeptidases PILS/ARTS1/ERAP1 and LNPEP/PLAP (1, 3). In addition to antigen presentation, the members of this subfamily are also important in maintenance of pregnancy, memory retention, and blood pressure regulation (3).

References

  1. Tanioka, T. et al. (2003) J. Biol. Chem. 278:32275.
  2. Tanioka, T. et al. (2005) FEBS J. 272:916.
  3. Tsujimoto, M. and A. Hattori (2005) Biochim. Biophys. Acta 1751:9.

Long Name

Leukocyte-derived Arginine Aminopeptidase

Alternate Names

ERAP2

Entrez Gene IDs

64167 (Human)

Gene Symbol

ERAP2

UniProt

Q6P179

Additional Aminopeptidase LRAP/ERAP2 Products

Product Documents for Human Aminopeptidase LRAP/ERAP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Aminopeptidase LRAP/ERAP2 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human Aminopeptidase LRAP/ERAP2 Antibody

Customer Reviews for Human Aminopeptidase LRAP/ERAP2 Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 24040622
    Species: Human
    Verified Customer | Posted 01/08/2015

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