Human AP-2 beta Antibody

Catalog # Availability Size / Price Qty
Detection of Human AP‑2 beta  by Western Blot.
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Product Details
Supplemental Products

Human AP-2 beta Antibody Summary

Species Reactivity
Detects endogenous human AP-2 beta in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human AP-2 beta
Accession # Q92481
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human AP‑2 beta  antibody by Western Blot. View Larger

Detection of Human AP‑2 beta by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human AP-2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5634) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AP-2 beta at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AP-2 beta

AP-2 beta (Activating enhancer-binding protein 2-beta; also TFAP2B) is a 52 kDa member of the AP-2 family of transcription factors. It is primarily a fetal-expressed protein, being found in renal and skin epithelial cells, as well as neuroblasts plus cells of the facial mesenchyme. In adults, AP-2 beta is expressed in renal epithelium. Human AP-2 beta is 460 amino acids (aa) in length. It contains a transactivation domain (aa 41‑131), a DNA-binding motif (aa 132‑298) and a C-terminal dimerization region (aa 299‑439). SUMOylation occurs on Lys21, adding 15 kDa of MW in SDS-PAGE. AP-2 beta both homodimerizes, and heterodimerizes with AP-2 alpha and -2 gamma. Dimers appear important for facial and limb development.

Long Name
Activating Enhancer-binding Protein 2 beta
Entrez Gene IDs
7021 (Human); 21419 (Mouse); 301285 (Rat)
Alternate Names
activating enhancer binding protein 2 beta; Activating enhancer-binding protein 2-beta; AP2 beta; AP-2 beta; AP-2B; AP2-B; AP2-beta; CHAR; MGC21381; TFAP2B; transcription factor AP-2 beta (activating enhancer binding protein 2 beta); transcription factor AP-2 beta (activating enhancer-binding protein 2 beta); transcription factor AP-2-beta

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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