Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AP-2 beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AP-2 beta
Leu121-Phe218
Accession # Q92481

Specificity

Detects endogenous human AP-2 beta in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human AP‑2 beta Antibody

Detection of Human AP‑2 beta antibody by Western Blot.

Detection of Human AP‑2 beta by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human AP-2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5634) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AP-2 beta at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human AP‑2 beta Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AP-2 beta

AP-2 beta (Activating enhancer-binding protein 2-beta; also TFAP2B) is a 52 kDa member of the AP-2 family of transcription factors. It is primarily a fetal-expressed protein, being found in renal and skin epithelial cells, as well as neuroblasts plus cells of the facial mesenchyme. In adults, AP-2 beta is expressed in renal epithelium. Human AP-2 beta is 460 amino acids (aa) in length. It contains a transactivation domain (aa 41‑131), a DNA-binding motif (aa 132‑298) and a C-terminal dimerization region (aa 299‑439). SUMOylation occurs on Lys21, adding 15 kDa of MW in SDS-PAGE. AP-2 beta both homodimerizes, and heterodimerizes with AP-2 alpha and -2 gamma. Dimers appear important for facial and limb development.

Long Name

Activating Enhancer-binding Protein 2 beta

Alternate Names

AP2 beta, CHAR, TFAP2B

Entrez Gene IDs

7021 (Human); 21419 (Mouse); 301285 (Rat)

Gene Symbol

TFAP2B

UniProt

Q92481

Additional AP-2 beta Products

Product Documents for Human AP‑2 beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human AP‑2 beta Antibody

For research use only

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