Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AP-2 epsilon Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AP-2 epsilon
Ala123-Val217
Accession # Q6VUC0

Specificity

Detects human AP-2 epsilon in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) AP‑2 alpha, rhAP‑2 beta, rhAP‑2 delta, and rhAP‑2 gamma is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human AP‑2 epsilon Antibody

Detection of Human AP‑2e antibody by Western Blot.

Detection of Human AP‑2 epsilon by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human AP-2e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for AP-2e at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human AP‑2 epsilon Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line and human placenta tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AP-2 epsilon

AP-2 epsilon (Activating protein 2 epsilon) is a 48 kDa member of the AP-2 transcription factor family. It is found in embryonic olfactory bulb mitral cells and adult keratinocytes and chondrocytes. In the nucleus, it presumably forms homodimers and heterodimers with other AP‑2 family members. Human AP‑2 epsilon is 442 amino acids in length. It contains a Gln/Pro-rich transactivation domain (aa 22‑122), a DNA binding domain (aa 216‑286) and an AP‑2, helix‑span‑helix dimerization region (aa 287‑417). There is one potential alternate start site at Met09. Over aa 123‑217, human AP‑2 epsilon shares 86% aa identity with mouse AP‑2 epsilon.

Long Name

Activating Enhancer-binding Protein epsilon

Alternate Names

AP2 epsilon, TFAP2E

Entrez Gene IDs

339488 (Human)

Gene Symbol

TFAP2E

UniProt

Q6VUC0

Additional AP-2 epsilon Products

Product Documents for Human AP‑2 epsilon Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human AP‑2 epsilon Antibody

For research use only

Citations for Human AP‑2 epsilon Antibody

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