Detects human AP-2 epsilon in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) AP‑2 alpha, rhAP‑2 beta, rhAP‑2δ, and rhAP‑2 gamma is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human AP-2 epsilon Ala123-Val217 Accession # Q6VUC0
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human AP‑2 epsilon by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human AP‑2 epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for AP‑2 epsilon at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AP-2 epsilon
AP-2 epsilon (Activating protein 2 epsilon) is a 48 kDa member of the AP-2 transcription factor family. It is found in embryonic olfactory bulb mitral cells and adult keratinocytes and chondrocytes. In the nucleus, it presumably forms homodimers and heterodimers with other AP‑2 family members. Human AP‑2 epsilon is 442 amino acids in length. It contains a Gln/Pro-rich transactivation domain (aa 22‑122), a DNA binding domain (aa 216‑286) and an AP‑2, helix‑span‑helix dimerization region (aa 287‑417). There is one potential alternate start site at Met09. Over aa 123‑217, human AP‑2 epsilon shares 86% aa identity with mouse AP‑2 epsilon.
Activating Enhancer-binding Protein epsilon
Entrez Gene IDs:
Activating enhancer-binding protein 2-epsilon; adaptor-related protein complex 2, epsilon subunit; AP2 epsilon; AP-2 epsilon; AP2E; AP2-epsilon; MGC49007; TFAP2E; transcription factor AP-2 epsilon (activating enhancer binding protein 2epsilon); transcription factor AP-2-epsilon
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