Human Apolipoprotein A-I/ApoA1 Antibody

R&D Systems | Catalog # AF3664

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Apolipoprotein A-I/ApoA1 Primary Antibodies »

Product Specifications

Immunogen

Human Plasma ApoA1 and E. coli-derived recombinant human ApoA1
Asp25-Gln267
Accession # P02647

Specificity

Detects human ApoA1 in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human (rh) ApoA2, rhApoB100, and rhApoC2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Apolipoprotein A-I/ApoA1 Antibody

Detection of Human Apolipoprotein A-I/ApoA1 antibody by Western Blot.

Detection of Human Apolipoprotein A‑I/ApoA1 by Western Blot.

Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Apolipoprotein A-I/ApoA1 antibody by Simple WesternTM.

Detection of Human Apolipoprotein A‑I/ApoA1 by Simple WesternTM.

Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 32 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Apolipoprotein A-I by Western Blot

Detection of Apolipoprotein A-I by Western Blot

Purity assessment ofEVs isolated by nine methods using SimpleWestern. (A) Comparison of EV markers and contaminant proteins inthe nine EV isolation methods. Sample loading was normalized to thesame starting plasma volume. Neat Plasma, HansaBioMed plasma EV (P-EV),and cell EV (C EV) standards were used as controls. (B) Reproducibilityof EV preparation by measuring CD9 (n = 3, mean ±SD and CV%). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40356199), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Apolipoprotein A-I/ApoA1 Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: Human serum

Western Blot

0.5 µg/mL
Sample: Human Plasma

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Apolipoprotein A-I/ApoA1

ApoA1 is a major protein component of serum high-density lipoprotein (HDL) and is produced by the liver and small intestine. It is involved in reverse cholesterol transport from tissues to the liver. ApoA1 is synthesized as a 267 amino acid (aa) precursor from which a signal peptide and short propeptide are removed. Mature human ApoA1 shares 65% and 61% aa sequence identity with mouse and rat ApoA1, respectively.

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Entrez Gene IDs

335 (Human); 11806 (Mouse); 25081 (Rat)

Gene Symbol

APOA1

UniProt

P02647

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Human Apolipoprotein A-I/ApoA1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Apolipoprotein A-I/ApoA1 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Obesity

Citations for Human Apolipoprotein A-I/ApoA1 Antibody

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