Human Apolipoprotein A-I/ApoA1 Antibody Summary
Asp25-Gln267
Accession # P02647
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Apolipoprotein A‑I/ApoA1 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Apolipoprotein A‑I/ApoA1 by Simple WesternTM. Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 32 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein A-I/ApoA1
ApoA1 is a major protein component of serum high-density lipoprotein (HDL) and is produced by the liver and small intestine. It is involved in reverse cholesterol transport from tissues to the liver. ApoA1 is synthesized as a 267 amino acid (aa) precursor from which a signal peptide and short propeptide are removed. Mature human ApoA1 shares 65% and 61% aa sequence identity with mouse and rat ApoA1, respectively.
Product Datasheets
Citations for Human Apolipoprotein A-I/ApoA1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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Astrocyte- and Neuron-Derived Extracellular Vesicles from Alzheimer's Disease Patients Effect Complement-Mediated Neurotoxicity.
Authors: Nogueras-Ortiz, C J, Mahairaki, V Et al.
Cells
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FGF21 and APOA1 mRNA-based therapies for the treatment of experimental acute pancreatitis
Authors: Lopez-Pascual, A;Santamaria, E;Ardaiz, N;Uriarte, I;Palmer, T;Graham, AR;Gomar, C;Barbero, RC;Latasa, MU;Arechederra, M;Urman, JM;Berasain, C;Fontanellas, A;Del Rio, CL;Fernandez-Barrena, MG;Martini, PGV;Schultz, JR;Berraondo, P;Avila, MA;
Journal of translational medicine
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Host biomarker-based quantitative rapid tests for detection and treatment monitoring of tuberculosis and COVID-19
Authors: Pierneef L, van Hooij A, de Jong D et al.
iScience
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Multi-omic profiling of the leukemic microenvironment shows bone marrow interstitial fluid is distinct from peripheral blood plasma
Authors: Lorenz Nierves, Jian Guo, Siyuan Chen, Janice Tsui, Anuli C. Uzozie, Jonathan W. Bush et al.
Experimental Hematology & Oncology
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Neuronal-Derived EV Biomarkers Track Cognitive Decline in Alzheimer’s Disease
Authors: Erden Eren, Jeannie-Marie Leoutsakos, Juan Troncoso, Constantine G. Lyketsos, Esther S. Oh, Dimitrios Kapogiannis
Cells
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Mitochondrial Electron Transport Chain Protein Abnormalities Detected in Plasma Extracellular Vesicles in Alzheimer's Disease
Authors: PJ Yao, E Eren, EJ Goetzl, D Kapogianni
Biomedicines, 2021-10-31;9(11):.
Species: Human
Sample Types: Extracellular
Applications: Western Blot -
Uncovering temperature‐dependent extracellular vesicle secretion in breast cancer
Authors: Kurataka Otsuka, Yusuke Yamamoto, Takahiro Ochiya
Journal of Extracellular Vesicles
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Serum amyloid A is a soluble pattern recognition receptor that drives type 2 immunity
Authors: U Smole, N Gour, J Phelan, G Hofer, C Köhler, B Kratzer, PA Tauber, X Xiao, N Yao, J Dvorak, L Caraballo, L Puerta, S Rosskopf, J Chakir, E Malle, AP Lane, WF Pickl, S Lajoie, M Wills-Karp
Nat. Immunol., 2020-06-22;21(7):756-765.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Immunoprecipitation -
Adenosine leakage from perforin-burst extracellular vesicles inhibits perforin secretion by cytotoxic T-lymphocytes
Authors: H Tadokoro, A Hirayama, R Kudo, M Hasebe, Y Yoshioka, J Matsuzaki, Y Yamamoto, M Sugimoto, T Soga, T Ochiya
PLoS ONE, 2020-04-10;15(4):e0231430.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Application of new host biomarker profiles in quantitative point-of-care tests facilitates leprosy diagnosis in the field
Authors: Anouk van Hooij, Susan van den Eeden, Renate Richardus, Elisa Tjon Kon Fat, Louis Wilson, Kees L.M.C. Franken et al.
EBioMedicine
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Aspirin hydrolysis in plasma is a variable function of butyrylcholinesterase and platelet-activating factor acetylhydrolase 1b2 (PAFAH1b2).
Authors: Zhou G, Marathe G, Hartiala J, Hazen S, Allayee H, Tang W, McIntyre T
J Biol Chem, 2013-03-18;288(17):11940-8.
Species: Human
Sample Types: Plasma
Applications: Western Blot
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