Human Attractin Antibody

Catalog # Availability Size / Price Qty
MAB7238
MAB7238-SP
Attractin in HepG2 Human Cell Line.
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Human Attractin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Attractin in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 750003
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Attractin
Ala84-Gln1272
Accession # O75882
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Attractin antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

Attractin in HepG2 Human Cell Line. Attractin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Attractin Monoclonal Antibody (Catalog # MAB7238) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Attractin

Attractin (ATRN), also known as DPPT-L, is an approximately 200 kDa transmembrane glycoprotein that shows dipeptidyl peptidase activity similar to DPPIV/CD26. Attractin is involved in a variety of processes including monocyte-T cell adhesion, axon myelination, melanocyte pigment synthesis, and energy homeostasis. The extracellular region of human Attractin contains one EGF-like domain, one CUB domain, six Kelch repeats, four PSI domains, one C-type lectin domain, and two laminin EGF-like domains. Alternate splicing of human Attractin generates a secreted isoform that lacks the transmembrane and cyotplasmic regions. Attractin is transiently upregulated during T cell activation before expression switches to the 175 kDa secreted isoform which is released into the circulation. Soluble Attractin is preferentially expressed by leukocytes and differentiating neurons. It blocks neurite formation and is elevated in the CSF of astrocytoma patients. Within aa 84-1272, human Attractin shares 95% aa sequence identity with mouse and rat Attractin.

Entrez Gene IDs
8455 (Human); 11990 (Mouse); 83526 (Rat)
Alternate Names
ATRN; Attractin; DPPT-L; KIAA0548; Mahogany Protein; MGC126754; MGCA

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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