Human beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody

Catalog # Availability Size / Price Qty
MAB36091-100
MAB36091-SP
 beta -1,4‑Galactosyltransferase 1/B4GalT1 in HeLa Human Cell Line.
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Human beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta -1,4‑Galactosyltransferase 1/B4GalT1in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 394709
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human beta -1,4‑Galactosyltransferase 1/B4GalT1
Ala44-Ser398
Accession # P15291
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry beta -1,4-Galactosyltransferase 1/B4GalT1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

beta -1,4‑Galactosyltransferase 1/B4GalT1 in HeLa Human Cell Line. beta -1,4-Galactosyltransferase 1/B4GalT1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human beta -1,4-Galactosyltransferase 1/B4GalT1 Monoclonal Antibody (Catalog # MAB36091) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1,4-Galactosyltransferase 1/B4GalT1

beta 4GalT1 is one of seven beta 1,4 galactosyltransferases that transfer galactose in a beta 1,4 linkage to acceptor sugars including GlcNAc, and Glc, and Xyl. By sequence similarity, the beta 4GalTs form four groups: beta 4GalT1 and beta 4GalT2, beta 4GalT3 and beta 4GalT4, beta 4GalT5 and beta 4GalT6, and beta 4GalT7 (1). beta 4GalT1 is unique among the seven enzymes because it can be expressed either as membrane associated form or secreted form (2). The secreted form is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha -lactalbumin to catalyze the synthesis of lactose (3). The membrane form can reside either in the Golgi apparatus, where it adds galactose to N-acetylglucosamine residues, or on cell surface, where it functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix (4). The two enzymatic forms result from alternate transcription initiation sites and post-translational processing (5). Defects in beta 4GalT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D) (6). The amino acid sequence of human B4GALT1 is 88%, 87% and 71% identical to that of rat, mouse/canine and chicken.

References
  1. Amado, M. et al. (1999) Biochim. Biophys. Acta. 1473:35.
  2. Yamaguchi, N and Fukuda, M.N. (1995) J. Biol. Chem. 270:12170.
  3. Appert, H.E. et al. (1986) Biochem. Biophys. Res. Commun. 138:224.
  4. Lopez, L.C. et al. (1991) J. Biol. Chem. 266:15984.
  5. Mengle-Gaw, L. et al. (1991) Biochem. Biophys. Res. Commun. 176:1269.
  6. Hansske, B. et al. (2002) J. Clin. Invest. 109:725.
Long Name
UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, Polypeptide 1
Entrez Gene IDs
2683 (Human); 14595 (Mouse); 24390 (Rat)
Alternate Names
1,4- galactosyltransferase, polypeptide 1; B4GalT1; B4GAL-T1; beta-1,4-galactosyltransferase 1; Beta-1,4-GalTase 1; beta4Gal-T1; betaGlcNAc beta; CDG2D; EC 2.4.1; GGTB2; GGTB2DKFZp686N19253; glycoprotein-4-beta-galactosyltransferase 2; GT1; GTB; lactose synthase; MGC50983; UDP-Gal; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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