Human Bora Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Bora in direct ELISAs.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Bora
    Gly2-Ala180
    Accession # Q6PGQ7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Bora in MCF‑7 Human Cell Line. Bora was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human Bora Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6856) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bora

Bora (Aurora borealis; also C13orf34) is a 61 kDa member of the Bora family of proteins. It is ubiquitously expressed, and plays a key role in cell cycle progression. Plk1 (polo-like kinase-1) is a phosphorylase that is important to the cell during the G2/M transition and mitosis. Its activity is initially regulated by Aurora-A, which phosphorylates and activates Plk1 on Thr210. Bora, Aurora-A and Plk1 all appear to form a complex during G2. Bora predisposes Plk1 to the actions of Aurora-A. Once activated by Aurora-A, Plk1 drives the mitotic mechanism, which includes a third-party phosphorylation of Bora. This initiates Bora dissociation from Aurora-A with subsequent ubiquitination and degradation. Human Bora is 559 amino acids (aa) in length. It contains a Ser-rich region (aa 188-278) and at least eight utilized Ser phosphorylation sites. Phosphorylation may increase the SDS-PAGE MW of Bora to 75-85 kDa. There is one potential alternative start site that lies 60 aa upstream of the standard start site, and a second splice variant the shows a 17 aa substitution for aa 1-87. Over aa 2-180, human Bora shares 84% aa identity with mouse Bora.

  • Long Name:
    Protein Aurora Borealis
  • Entrez Gene IDs:
    79866 (Human); 777441 (Mouse); 306102 (Rat)
  • Alternate Names:
    Aurora Borealis; Bora; C13orf34; chromosome 13 open reading frame 34; FLJ22624; hsBora; protein aurora borealis
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