Human Cadherin-11 Antibody (667039)

R&D Systems | Catalog # MAB17901

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 667039
View all Cadherin-11 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cadherin-11
Phe23-Thr617
Accession # AAA35622

Specificity

Detects human Cadherin-11 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Cadherin-4, -6, -8, -12, -13,-17, rhE-Cadherin, rhN-Cadherin, rhP-Cadherin, or rhVE-Cadherin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Cadherin-11 Antibody (667039)

Detection of Cadherin-11 antibody in PC-3 Human Cell Line antibody by Flow Cytometry.

Detection of Cadherin‑11 in PC‑3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Cadherin-11 Monoclonal Antibody (Catalog # MAB17901, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Cells were stained in a buffer containing Ca2+and Mg2+.

Applications for Human Cadherin-11 Antibody (667039)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: PC‑3 human prostate cancer cell line stained in buffer containing Ca2+ and Mg2+

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cadherin-11

Cadherin-11, also known as OB-Cadherin, is a 120 kDa member of the classical Cadherin family of calcium-dependent homophilic adhesion proteins. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1). Cadherin-11 is expressed in embryonic mesodermal tissues and contributes to the morphogenesis of the nervous and skeletal systems (2-4). It is expressed on osteoblasts in the adult where it promotes the differentiation of both osteoblasts and chondrocytes (5). Cadherin-11 is up‑regulated on breast cancer and prostate cancer cells which preferentially metastasize to bone (6, 7). It facilitates this metastasis via homophilic adhesion to bone marrow stroma and osteoblast-expressed Cadherin-11 (6‑8). In the synovium, Cadherin‑11 supports adhesion between synoviocytes but promotes cell invasion in synovitis and rheumatoid arthritis (9, 10). Its up‑regulation in the vasculature following injury contributes to intimal hyperplasia by inducing smooth muscle cell migration and proliferation (11). In the nervous system, Cadherin-11 interacts with FGF R1 to promote neurite extension from spinal cord explants (12). Mature human Cadherin‑11 consists of a 564 amino acid (aa) extracellular domain (ECD) with five tandem Cadherin repeats, a 23 aa transmembrane segment, and a 156 aa cytoplasmic domain (13, 14). Within the ECD, human Cadherin-11 shares 97% and 98% aa sequence identity with mouse and rat Cadherin‑11, respectively. An 80 kDa portion of the Cadherin‑11 ECD can be shed by proteolytic cleavage, and this fragment competes with the full length molecule for cell adhesion (3, 15). Alternate splicing of human Cadherin-11 generates an 85 kDa isoform with substituted transmembrane and cytoplasmic regions (14, 15).

References

  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Kimura, Y. et al. (1995) Dev. Biol. 169:347.
  3. McCusker, C. et al. (2009) Mol. Biol. Cell 20:78.
  4. Clendenon, S.G. et al. (2009) Dev. Dyn. 238:1909.
  5. Kii, I. et al. (2004) J. Bone Mineral Res. 19:1840.
  6. Tamura, D. et al. (2008) Int. J. Oncol. 33:17.
  7. Chu, K. et al. (2008) Mol. Cancer Res. 6:1259.
  8. Huang, C.-F. et al. (2010) Cancer Res. 70:4580.
  9. Valencia, X. et al. (2004) J. Exp. Med. 200:1673.
  10. Kiener, H.P. et al. (2009) Arthritis Rheum. 60:1305.
  11. Monahan, T.S. et al. (2007) J. Vasc. Surg. 45:581.
  12. Boscher, C. and R.-M. Mege (2008) Cell. Signal. 20:1061.
  13. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
  14. Okazaki, M. et al. (1994) J. Biol. Chem. 269:12092.
  15. Kawaguchi, J. et al. (1999) J. Bone Mineral Res. 14:764.

Alternate Names

Cadherin11, CDH11, CDHOB, OB-Cadherin

Entrez Gene IDs

1009 (Human); 12552 (Mouse)

Gene Symbol

CDH11

UniProt

AAA35622

Additional Cadherin-11 Products

Product Documents for Human Cadherin-11 Antibody (667039)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cadherin-11 Antibody (667039)

For research use only

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

Citations for Human Cadherin-11 Antibody (667039)

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FAQs for Human Cadherin-11 Antibody (667039)

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  • Q: Why does the staining protocol with this Cadherin antibody use buffers containing Ca2+ and Mg2+?

    A: The staining protocol with this and other Cadherin antibodies uses buffer containing Ca2+ and Mg2+ because Cadherin function is Calcium-dependent. 

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