Human Carbohydrate Sulfotransferase 2/CHST2 Antibody

R&D Systems | Catalog # AF5107

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Carbohydrate Sulfotransferase 2/CHST2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CHST2
Tyr76-Leu530
Accession # Q9Y4C5

Specificity

Detects human Carbohydrate Sulfotransferase 2/CHST2 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CHST1, rhCHST5, and recombinant mouse CHST7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Carbohydrate Sulfotransferase 2/CHST2 Antibody

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbohydrate Sulfotransferase 2/CHST2

The CHST family is comprised of 14 genes in both human and mouse. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). Human CHST2, also known as N-acetylglucosamine-6-O-sulfotransferase 1 (GlcNAc6ST-1) and Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST-2), was previously shown to act on non-reducing GlcNAc residues (3). The enzyme is known to be involved in biosynthesis of L-selectin ligand sialyl 6-sulfo Lewis X (4) and therefore plays a role in lymphocyte homing (5).

References

  1. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
  2. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
  3. Sakaguchi, H. et al. (2000) Biochim. Biophys. Acta 1523:269.
  4. Uchimura, K. et al. (1998) J. Biol. Chem. 273:22577.
  5. Li, X. et al. (2001) J. Leukoc. Biol. 69:565.
  6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, p.964.
  7. MacRae, I.J. et al. (2000) Biochemistry 39:1613.
  8. Wu, Z.L. et al. (2002) FASEB J. 16:539.

Alternate Names

C6ST, CHST2, GlcNAc6ST-1, GN6ST, GST-2

Entrez Gene IDs

9435 (Human); 54371 (Mouse)

Gene Symbol

CHST2

UniProt

Q9Y4C5

Additional Carbohydrate Sulfotransferase 2/CHST2 Products

Product Documents for Human Carbohydrate Sulfotransferase 2/CHST2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Carbohydrate Sulfotransferase 2/CHST2 Antibody

For research use only

Citations for Human Carbohydrate Sulfotransferase 2/CHST2 Antibody

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Associated Pathways

Articular Cartilage Extracellular Matrix
Articular Cartilage Extracellular Matrix Thumbnail