Human Carbohydrate Sulfotransferase 2/CHST2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Carbohydrate Sulfotransferase 2/CHST2 by Western Blot.
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Product Details
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Human Carbohydrate Sulfotransferase 2/CHST2 Antibody Summary

Species Reactivity
Detects human Carbohydrate Sulfotransferase 2/CHST2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) CHST1, rhCHST5, and recombinant mouse CHST7 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant human CHST2
Accession # Q9Y4C5
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Carbohydrate Sulfotransferase 2/CHST2 (Catalog # 5107-ST), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Carbohydrate Sulfotransferase 2/CHST2 antibody by Western Blot. View Larger

Detection of Human Carbohydrate Sulfotransferase 2/CHST2 by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbohydrate Sulfotransferase 2/CHST2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5107) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbohydrate Sulfotransferase 2/CHST2 at approximately 50- 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 2/CHST2

The CHST family is comprised of 14 genes in both human and mouse. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). Human CHST2, also known as N-acetylglucosamine-6-O-sulfotransferase 1 (GlcNAc6ST-1) and Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST-2), was previously shown to act on non-reducing GlcNAc residues (3). The enzyme is known to be involved in biosynthesis of L-selectin ligand sialyl 6-sulfo Lewis X (4) and therefore plays a role in lymphocyte homing (5).

  1. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
  2. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
  3. Sakaguchi, H. et al. (2000) Biochim. Biophys. Acta 1523:269.
  4. Uchimura, K. et al. (1998) J. Biol. Chem. 273:22577.
  5. Li, X. et al. (2001) J. Leukoc. Biol. 69:565.
  6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, p.964.
  7. MacRae, I.J. et al. (2000) Biochemistry 39:1613.
  8. Wu, Z.L. et al. (2002) FASEB J. 16:539.
Entrez Gene IDs
9435 (Human); 54371 (Mouse)
Alternate Names
C6ST; carbohydrate (chondroitin 6/keratan) sulfotransferase 2; carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2; Carbohydrate Sulfotransferase 2; CHST2; EC 2.8.2.-; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2; GlcNAc6ST-1; GN6ST; Gn6ST-1; GST2; GST-2; N-acetylglucosamine 6-O-sulfotransferase 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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