Human Carbonic Anhydrase VI Antibody

Catalog # Availability Size / Price Qty
AF2939
AF2939-SP
Detection  of Human Carbonic Anhydrase VI by Western Blot.
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Human Carbonic Anhydrase VI Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carbonic Anhydrase VI in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Carbonic Anhydrase (CA) 5A and rhCA5B is observed and less than 1% cross-reactivity with rhCA1, rhCA2, rhCA3, rhCA4, rhCA7, rhCA8, rhCA9, rhCA10, rhCA12, rhCA13, and rhCA14 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase VI
Gln18-Asn308
Accession # EAW71606
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VI (Catalog # 2939-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human Carbonic Anhydrase VI by Western Blot. View Larger

Detection of Human Carbonic Anhydrase VI by Western Blot. Western blot shows human saliva, human milk, and lysates of HSC-3 human oral squamous cell carcinoma cell line and LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Carbonic Anhydrase VI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2939) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase VI at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection  of Human Carbonic Anhydrase VI by Simple  WesternTM. View Larger

Detection of Human Carbonic Anhydrase VI by Simple WesternTM. Simple Western lane view shows lysates of human saliva, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase VI at approximately 56 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Carbonic Anhydrase VI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2939) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase VI

Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc-metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5).

References
  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Murakami, H. and Sly, W. S. (1987) J. Biol. Chem. 262:1382.
  3. Thatcher, B. J. et al. (1998) Biochem. Biophys. Res. Commun. 250:635.
  4. Hankin, R. I. et al. (1999) Am. J. Med. Sci. 318:380.
  5. Karhumaa, P. et al. (2001) Proc. Natl. Acad. Sci. USA. 98:11604.
Entrez Gene IDs
765 (Human); 298657 (Rat)
Alternate Names
CA6; Carbonate dehydratase VI; carbonic anhydrase 6; Carbonic Anhydrase VI; carbonic anhydrase VIGUSTIN; CA-VI; EC 4.2.1.1; Gustin; MGC21256; Salivary carbonic anhydrase; Secreted carbonic anhydrase

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Reconstitution Buffers

Secondary Antibodies

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