Human Carbonic Anhydrase XII/CA12 Antibody Summary
Accession # O43570
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Carbonic Anhydrase XII/CA12 by Western Blot.
Western blot shows lysates of human kidney tissue and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase XII/CA12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2190) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase XII/CA12 at approximately
45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Carbonic Anhydrase XII/CA12 by Simple WesternTM.
Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase XII/CA12 at approximately 60 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Carbonic Anhydrase XII/CA12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2190) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase XII/CA12
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA12 is a type I membrane enzyme highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis (2, 3). It is over-expressed in some renal cell cancers and in glaucoma (2, 4). Two alternatively spliced forms exist, which either contains or lacks a 11 amino acid segment just before the transmembrane domain (5). The secreted, purified recombinant human CA12 contains the residues that are common for both forms.
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Tuereci, O. et al. (1998) Proc. Natl. Acad. Sci. USA 95:7608.
- Ivanov, S.V. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12596.
- Liao, S.-Y. et al. (2003) J. Med. Genet. 40:257.
- Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
Citation for Human Carbonic Anhydrase XII/CA12 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Carbonic anhydrase IX promotes tumor growth and necrosis in vivo and inhibition enhances anti-VEGF therapy.
Authors: McIntyre A, Patiar S, Wigfield S
Clin. Cancer Res., 2012;18(11):3100-11.
Sample Types: Cell Lysates
Applications: Western Blot
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