Human Carbonic Anhydrase XII/CA12 Antibody Summary
Ala25-Gln291
Accession # O43570
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Carbonic Anhydrase XII/CA12 by Western Blot. Western blot shows lysates of human kidney tissue and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase XII/CA12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2190) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase XII/CA12 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Carbonic Anhydrase XII/CA12 by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase XII/CA12 at approximately 60 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Carbonic Anhydrase XII/CA12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2190) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Carbonic Anhydrase XII/CA12 by Western Blot Differential expression of CAIX and CAXII in patient-derived tumor grafts. Panel A. Frozen tissue samples from PDX tumors were homogenized in RIPA buffer containing protease inhibitor. Western blots were probed with antibodies for CAIX, CAXII, CAII, ER, E-cadherin, actin, and GAPDH. Panel B. Tumor growth rates of orthotopically implanted, cryo-preserved tumor tissue was evaluated in NOD/SCID mice. Panel C. Immunohistochemistry was utilized to evaluate expression of CAIX, CAXII, ER, and Ki67 in TNBC (HCl-001) and ER/PR-positive (HCl-011) tumors from Panel B. Magnification: primary objective magnification, 10x. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29965974), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Carbonic Anhydrase XII/CA12 by Western Blot Differential expression of CAs in TNBC and luminal breast cancer cells. Cell were grown to 70% confluence and then exposed to normoxic (N) or 1% oxygen (H), hypoxic conditions. After 16 h, cells were washed with PBS and extracted in RIPA buffer containing protease inhibitors. Equal protein was loaded onto SDS PAGE gels, and then transferred to nitrocellulose for western blot analysis. Panel A. CA expression in TNBC cells: UFH = UFH-001, MDA = MDA-MB-231-LM2, HBL = HBL-100, S159 = Sum 159, BT = BT-549 cells. Panel B. CA expression in luminal breast cancer cells: T47D, MCF7 = MCF-7, SKBR = SKBR-3, S52 = SUM-52 cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29965974), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase XII/CA12
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA12 is a type I membrane enzyme highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis (2, 3). It is over-expressed in some renal cell cancers and in glaucoma (2, 4). Two alternatively spliced forms exist, which either contains or lacks a 11 amino acid segment just before the transmembrane domain (5). The secreted, purified recombinant human CA12 contains the residues that are common for both forms.
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Tuereci, O. et al. (1998) Proc. Natl. Acad. Sci. USA 95:7608.
- Ivanov, S.V. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12596.
- Liao, S.-Y. et al. (2003) J. Med. Genet. 40:257.
- Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
Product Datasheets
Citations for Human Carbonic Anhydrase XII/CA12 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Differential expression and function of CAIX and CAXII in breast cancer: A comparison between tumorgraft models and cells.
Authors: Chen Z, Ai L, Mboge MY et al.
PLoS ONE.
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Carbonic anhydrase IX promotes tumor growth and necrosis in vivo and inhibition enhances anti-VEGF therapy.
Authors: McIntyre A, Patiar S, Wigfield S
Clin. Cancer Res., 2012-04-12;18(11):3100-11.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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