Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Carbonic Anhydrase XII/CA12 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase XII/CA12
Ala25-Gln291
Accession # O43570

Specificity

Detects human Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse Carbonic Anhydrase XII/CA12 is observed and less than 5% cross-reactivity with recombinant human CA1, 3, 5A, 5B, 6, 7, 8, and 10 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Carbonic Anhydrase XII/CA12 Antibody

Detection of Human Carbonic Anhydrase XII/CA12 antibody by Western Blot.

Detection of Human Carbonic Anhydrase XII/CA12 by Western Blot.

Western blot shows lysates of human kidney tissue and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase XII/CA12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2190) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase XII/CA12 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Carbonic Anhydrase XII/CA12 antibody by Simple WesternTM.

Detection of Human Carbonic Anhydrase XII/CA12 by Simple WesternTM.

Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase XII/CA12 at approximately 60 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Carbonic Anhydrase XII/CA12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2190) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Carbonic Anhydrase XII/CA12 by Western Blot

Detection of Carbonic Anhydrase XII/CA12 by Western Blot

Differential expression of CAIX and CAXII in patient-derived tumor grafts. Panel A. Frozen tissue samples from PDX tumors were homogenized in RIPA buffer containing protease inhibitor. Western blots were probed with antibodies for CAIX, CAXII, CAII, ER, E-cadherin, actin, and GAPDH. Panel B. Tumor growth rates of orthotopically implanted, cryo-preserved tumor tissue was evaluated in NOD/SCID mice. Panel C. Immunohistochemistry was utilized to evaluate expression of CAIX, CAXII, ER, and Ki67 in TNBC (HCl-001) and ER/PR-positive (HCl-011) tumors from Panel B. Magnification: primary objective magnification, 10x. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29965974), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Carbonic Anhydrase XII/CA12 by Western Blot

Detection of Carbonic Anhydrase XII/CA12 by Western Blot

Differential expression of CAs in TNBC and luminal breast cancer cells. Cell were grown to 70% confluence and then exposed to normoxic (N) or 1% oxygen (H), hypoxic conditions. After 16 h, cells were washed with PBS and extracted in RIPA buffer containing protease inhibitors. Equal protein was loaded onto SDS PAGE gels, and then transferred to nitrocellulose for western blot analysis. Panel A. CA expression in TNBC cells: UFH = UFH-001, MDA = MDA-MB-231-LM2, HBL = HBL-100, S159 = Sum 159, BT = BT-549 cells. Panel B. CA expression in luminal breast cancer cells: T47D, MCF7 = MCF-7, SKBR = SKBR-3, S52 = SUM-52 cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29965974), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Carbonic Anhydrase XII/CA12 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: A549 human lung carcinoma cell line

Western Blot

1 µg/mL
Sample: Human kidney tissue and A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbonic Anhydrase XII/CA12

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA12 is a type I membrane enzyme highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis (2, 3). It is over-expressed in some renal cell cancers and in glaucoma (2, 4). Two alternatively spliced forms exist, which either contains or lacks a 11 amino acid segment just before the transmembrane domain (5). The secreted, purified recombinant human CA12 contains the residues that are common for both forms.

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Tuereci, O. et al. (1998) Proc. Natl. Acad. Sci. USA 95:7608.
  3. Ivanov, S.V. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12596.
  4. Liao, S.-Y. et al. (2003) J. Med. Genet. 40:257.
  5. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.

Alternate Names

CA12

Entrez Gene IDs

771 (Human); 76459 (Mouse)

Gene Symbol

CA12

UniProt

O43570

Additional Carbonic Anhydrase XII/CA12 Products

Product Documents for Human Carbonic Anhydrase XII/CA12 Antibody

Certificate of Analysis

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Product Specific Notices for Human Carbonic Anhydrase XII/CA12 Antibody

For research use only

Citations for Human Carbonic Anhydrase XII/CA12 Antibody

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