Detects human Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse Carbonic Anhydrase XII/CA12 is observed and less than 5% cross-reactivity with recombinant human CA1, 3, 5A, 5B, 6, 7, 8, and 10 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase XII/CA12 Ala25-Gln291 Accession # O43570
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human Carbonic Anhydrase XII/CA12 by Western Blot.
Western blot shows lysates of human kidney tissue and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase XII/CA12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2190) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase XII/CA12 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Carbonic Anhydrase XII/CA12 by Simple WesternTM.
Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase XII/CA12 at approximately 60 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Carbonic Anhydrase XII/CA12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2190) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase XII/CA12
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA12 is a type I membrane enzyme highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis (2, 3). It is over-expressed in some renal cell cancers and in glaucoma (2, 4). Two alternatively spliced forms exist, which either contains or lacks a 11 amino acid segment just before the transmembrane domain (5). The secreted, purified recombinant human CA12 contains the residues that are common for both forms.
Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Tuereci, O. et al. (1998) Proc. Natl. Acad. Sci. USA 95:7608.
Ivanov, S.V. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12596.
Liao, S.-Y. et al. (2003) J. Med. Genet. 40:257.
Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Have you used Human Carbonic Anhydrase XII/CA12 Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.