Detects human Cardiotrophin‑1/CT‑1 in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse Cardiotrophin-1 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human Cardiotrophin-1/CT-1 Ser2-Ala201 Accession # Q16619
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human Cardiotrophin‑1/CT‑1 (Catalog # 612-CD)
Measured by its ability to neutralize Cardiotrophin‑1/CT‑1-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.1‑0.3 µg/mL in the presence of 15 ng/mL Recombinant Human Cardiotrophin‑1/CT‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by Cardiotrophin‑1/CT‑1 and Neutralization by Human Cardiotrophin‑1/CT‑1 Antibody.
Recombinant Human Cardiotrophin‑1/CT‑1 (Catalog # 612-CD) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Cardiotrophin‑1/CT‑1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Cardiotrophin‑1/CT‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF612). The ND50 is typically 0.1-0.3 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has been implicated in the receptor complex.
Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81.
Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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