Human Caspase-10 Antibody Summary
Accession # AAC50644
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Caspase‑10 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Human FAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126) for for the indicated times. PVDF membrane was probed with 1 µg/mL of Human Caspase‑10 Monoclonal Antibody (Catalog # MAB834), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caspase‑10 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Caspase-10 (Cysteine-aspartic acid protease 10, FLICE2 and Mch4) is a 28-29 kDa member of the peptidase C14A family of enzymes. It is widely expressed, being found in blood and epithelial cells. Caspase-10 appears to be an initiator caspase that acts on Pro-Caspases-3, -4, -6, -7, and -9. Human Pro-Caspase-10a is a 59 kDa, 521 amino acid (aa) protein. It contains two N-terminal death domains. Cleavage generates p43 plus the small mature p12 subunit. Cleavage of p43 generates a prodomain p25 and large mature p17 subunit. P17 and p12 noncovalently associate to form a 29 kDa mature heterodimer. There are at least four isoform variants. There is no apparent mouse Casp-10 gene.
Citations for Human Caspase-10 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Identification of caspase-10 in human neutrophils and its role in spontaneous apoptosis.
Authors: Goepel F, Weinmann P, Schymeinsky J, Walzog B
J. Leukoc. Biol., 2004;75(5):836-43.
Sample Types: Cell Lysates
Upregulation of the Fas receptor death-inducing signaling complex after traumatic brain injury in mice and humans.
Authors: Qiu J, Whalen MJ, Lowenstein P, Fiskum G, Fahy B, Darwish R, Aarabi B, Yuan J, Moskowitz MA
J. Neurosci., 2002;22(9):3504-11.
Sample Types: Tissue Homogenates
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