Human Cbx2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Cbx2 in ELISA.
  • Source
    Monoclonal Mouse IgG1 Clone # 880833
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Cbx2
    Lys131-His217
    Accession # Q14781
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Cbx2 in iPS2 Human Stem Cells. Cbx2 was detected in immersion fixed iPS2 human induced pluripotent stem cells, differentiated with 0.2 µM retinoic acid for 4 days (panels A and C) or undifferentiated (panels B and D), using Mouse Anti-Human Cbx2 Monoclonal Antibody (Catalog # MAB8098) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panels; Catalog # NL007) and counterstained with DAPI (blue, lower panels). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cbx2
Chromobox homolog 2 (Cbx2), also known as CDCA6 and M33, is an approximately 56 kDa protein component of the nuclear polycomb repressive complex 1 (PRC1). PRC1 regulates chromatin organization and gene transcription. Cbx2 interacts with methylated histone H3, and this is regulated by phosphorylation of Ser42 within the Cbx2 chromodomain. Cbx2 is required for male sex determination by activating expression of the Sry gene. It is also important for germ cell viability and meiotic chromosome synapsis. Within amino acids 131-217, human Cbx2 shares 94% and 92% aa sequence identity with mouse and rat Cbx2, respectively. Alternative splicing generates an additional isoform that is substituted and truncated following Lys96.
  • Long Name:
    Chromobox Homolog 2
  • Entrez Gene IDs:
    84733 (Human); 12416 (Mouse); 303730 (Rat)
  • Alternate Names:
    Cbx2; CDCA6; cell division cycle associated 6; chromobox homolog 2 (Drosophila Pc class); chromobox homolog 2 (Pc class homolog, Drosophila); chromobox homolog 2; chromobox protein homolog 2; M33; MGC10561; modifier 3; Pc class homolog; SRXY5
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