Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 880833
View all Cbx2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cbx2
Lys131-His217
Accession # Q14781

Specificity

Detects human Cbx2 in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Cbx2 Antibody

Cbx2 antibody in iPS2 Human Stem Cells by Immunocytochemistry (ICC).

Cbx2 in iPS2 Human Stem Cells.

Cbx2 was detected in immersion fixed iPS2 human induced pluripotent stem cells, differentiated with 0.2 µM retinoic acid for 4 days (panels A and C) or undifferentiated (panels B and D), using Mouse Anti-Human Cbx2 Monoclonal Antibody (Catalog # MAB8098) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panels; Catalog # NL007) and counterstained with DAPI (blue, lower panels). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human Cbx2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed iPS2 human induced pluripotent stem cells differentiated with 0.2 μM retinoic acid for 4 days

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cbx2

Chromobox homolog 2 (Cbx2), also known as CDCA6 and M33, is an approximately 56 kDa protein component of the nuclear polycomb repressive complex 1 (PRC1). PRC1 regulates chromatin organization and gene transcription. Cbx2 interacts with methylated histone H3, and this is regulated by phosphorylation of Ser42 within the Cbx2 chromodomain. Cbx2 is required for male sex determination by activating expression of the Sry gene. It is also important for germ cell viability and meiotic chromosome synapsis. Within amino acids 131-217, human Cbx2 shares 94% and 92% aa sequence identity with mouse and rat Cbx2, respectively. Alternative splicing generates an additional isoform that is substituted and truncated following Lys96.

Long Name

Chromobox Homolog 2

Alternate Names

CDCA6, M33, SRXY5

Entrez Gene IDs

84733 (Human); 12416 (Mouse); 303730 (Rat)

Gene Symbol

CBX2

UniProt

Q14781

Additional Cbx2 Products

Product Documents for Human Cbx2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cbx2 Antibody

For research use only

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