Key Product Details

Species Reactivity

Human

Applications

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 88119
View all CCL15/MIP-1 delta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL15/MIP-1 delta
Ser46-Ile113
Accession # Q16663

Specificity

Detects human CCL15/MIP‑1 delta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL15/MIP-1 delta Antibody

Chemotaxis Induced by CCL15/MIP‑1 delta and Neutral-ization by Human CCL15/ MIP‑1 delta Antibody.

Chemotaxis Induced by CCL15/MIP‑1 delta and Neutral-ization by Human CCL15/ MIP‑1 delta Antibody.

Recombinant Human CCL15/ MIP-1d (Catalog # 628-LK) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/ MIP-1d (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL15/MIP-1d Monoclonal Antibody (Catalog # MAB3631). The ND50 is typically 5-20 µg/mL.

Applications for Human CCL15/MIP-1 delta Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CCL15/MIP‑1 delta 92 aa (Catalog # 363-MG)

Neutralization

Measured by its ability to neutralize CCL15/MIP‑1 delta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 1 ng/mL Recombinant Human CCL15/MIP‑1 delta 68 aa.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL15/MIP-1 delta

CCL15, also called macrophage inflammatory protein 1 delta (MIP-1 delta and Leukotactin-1 (LKN-1), is a CC chemokine with two additional cysteine residues that form a third disulfide bond. CCL15 functions as a chemoattractant for monocytes, T cells, and eosinophils through interactions with CCR1. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1 delta is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells.

References

  1. Wang, W. et al. (1998) J. Clinical Immunol. 18:214.

Alternate Names

HCC-2, Leukotactin 1, MIP-1 delta, MIP-5, MIP1 delta, NCC-3

Entrez Gene IDs

6359 (Human)

Gene Symbol

CCL15

UniProt

Q16663

Additional CCL15/MIP-1 delta Products

Product Documents for Human CCL15/MIP-1 delta Antibody

Certificate of Analysis

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Product Specific Notices for Human CCL15/MIP-1 delta Antibody

For research use only

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