Human CCL2/JE/MCP-1 Antibody

(13 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL2/JE/MCP-1 in Western blots. Does not cross-react with recombinant human (rh) CCL1, 3, 4, 5, 7, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, rmCCL1, 2, 3, 4, 5, 6, 7, 9/10MIP-1 gamma, 11, 12, 17, 19, 20, 22, 24, 25, or rrCCL20.
  • Source
    Monoclonal Mouse IgG1 Clone # 24822
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CCL2/JE/MCP-1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.9-4.5 µg/mL in the presence of 0.15 µg/mL Recombinant Human CCL2/JE/MCP‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human CCL2/JE/MCP‑1 by Western Blot. Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP‑1 (Catalog #
279-MC), Recombinant Human CCL8/
MCP‑2 (Catalog # 281-CP), Recombinant Human CCL7/MCP‑3/MARC (Catalog # 282-P3), Recombinant Human CCL13/
MCP‑4 (Catalog # 327-P4), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE), and Recombinant Rat CCL2/JE/MCP‑1 (Catalog # 3144-JE). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB279) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/
MCP‑1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutralization by Human CCL2/JE/MCP‑1 Antibody. Recombinant Human CCL2/JE/
MCP‑1 (Catalog #279-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/JE/
MCP‑1 (0.15 µg/mL) is neutralized (green line) by increasing concentrations of
Mouse Anti-Human CCL2/JE/
MCP‑1 Monoclonal Antibody (Catalog # MAB279). The ND50 is typically 0.9-4.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL2/JE/MCP-1
CCL2 is a beta -chemokine that is also known as monocyte chemotactic protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes.
  • Entrez Gene IDs:
    6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
  • Alternate Names:
    CCL2; MCAF; MCP-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Continuous Delivery of Neutralizing Antibodies Elevate CCL2 Levels in Mice Bearing MCF10CA1d Breast Tumor Xenografts
    Authors: M Yao, C Smart, Q Hu, N Cheng
    Transl Oncol, 2017;10(5):734-743.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
    Authors: Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, Hashimoto N, Imagama S, Ishiguro N, Suzumura A, Ueda M, Furukawa K, Yamamoto A
    J Neurosci, 2015;35(6):2452-64.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  3. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012;209(10):1813-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells.
    Authors: Dagouassat M, Suffee N, Hlawaty H, Haddad O, Charni F, Laguillier C, Vassy R, Martin L, Schischmanoff PO, Gattegno L, Oudar O, Sutton A, Charnaux N
    Int. J. Cancer, 2010;126(5):1095-108.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
    Authors: Renaud SJ, Sullivan R, Graham CH
    Placenta, 2009;30(4):313-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
    Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
    J. Pathol., 2008;214(3):328-36.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  7. Induction of host chemotactic response by Encephalitozoon spp.
    Authors: Fischer J, West J, Agochukwu N, Suire C, Hale-Donze H
    Infect. Immun., 2007;75(4):1619-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
    Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
    Atherosclerosis, 2007;192(1):56-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
    Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
    Clin. Cancer Res., 2007;13(19):5719-28.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  10. Systemic inflammation in nonalcoholic fatty liver disease is characterized by elevated levels of CCL2.
    Authors: Haukeland JW, Damas JK, Konopski Z, Loberg EM, Haaland T, Goverud I, Torjesen PA, Birkeland K, Bjoro K, Aukrust P
    J. Hepatol., 2006;44(6):1167-74.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors.
    Authors: Penninckx F, Ectors N
    Exp. Cell Res., 2005;303(2):331-42.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  12. The CC chemokine ligand, CCL2/MCP1, participates in macrophage fusion and foreign body giant cell formation.
    Authors: Kyriakides TR, Foster MJ, Keeney GE, Tsai A, Giachelli CM, Clark-Lewis I, Rollins BJ, Bornstein P
    Am. J. Pathol., 2004;165(6):2157-66.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells.
    Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P
    J. Leukoc. Biol., 2004;75(3):504-14.
    Species: Human
    Sample Type:
    Application: Flow
Expand to show all 13 Citations
Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Average Rating: 4 (Based on 1 review)

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ELISA Human CCL2/JE/MCP-1 Antibody MAB279
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 ELISA Human Anonymous 11/20/2017
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ELISA Human CCL2/JE/MCP-1 Antibody MAB279
ELISA: Human CCL2/JE/MCP-1 Antibody [MAB279]

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThis antibody was used with BAF279 to create a sandwich ELISA to measure MCP-1 in human serum samples.

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