Detects human CCL2/JE/MCP-1 in Western blots. Does not cross-react with recombinant human (rh) CCL1, 3, 4,
5, 7, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, rmCCL1, 2, 3, 4, 5,
6, 7, 9/10MIP-1 gamma, 11, 12, 17, 19, 20, 22, 24, 25, or rrCCL20.
Monoclonal Mouse IgG1 Clone # 24822
Protein A or G purified from ascites
E. coli-derived recombinant human CCL2/JE/MCP-1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.9-4.5 µg/mL in the presence of 0.15 µg/mL Recombinant Human CCL2/JE/MCP‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Recombinant Human CCL2/JE/MCP‑1 by Western Blot. Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC), Recombinant Human CCL8/ MCP‑2 (Catalog # 281-CP), Recombinant Human CCL7/MCP‑3/MARC (Catalog # 282-P3), Recombinant Human CCL13/ MCP‑4 (Catalog # 327-P4), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE), and Recombinant Rat CCL2/JE/MCP‑1 (Catalog # 3144-JE). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB279) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/ MCP‑1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutralization by Human CCL2/JE/MCP‑1 Antibody. Recombinant Human CCL2/JE/ MCP‑1 (Catalog #279-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/JE/ MCP‑1 (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL2/JE/ MCP‑1 Monoclonal Antibody (Catalog # MAB279). The ND50 is typically 0.9-4.5 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCL2 is a beta -chemokine that is also known as monocyte chemotactic
protein 1 (MCP-1). It is produced by a variety of cell types and is
chemotactic for monocytes.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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