Detects human CCL28 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11,
12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1,
2, 3, 4, 5, 6, 7, 9, 11, 19, 21, 22, 25, or recombinant rat CCL20 is observed.
Monoclonal Mouse IgG1 Clone # 62705
Protein A or G purified from ascites
E. coli-derived recombinant human CCL28 Ile23-Tyr127 Accession # Q9NRJ3
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize CCL28-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR10. The Neutralization Dose (ND50) is typically 15-45 µg/mL in the presence of 4 µg/mL Recombinant Human CCL28.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody. Recombinant Human CCL28 (Catalog # 717-VC) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL28 (4 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL28 Monoclonal Antibody (Catalog # MAB717). The ND50 is typically 15‑45 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human CCL28 (CC chemokine ligand 28) is a 127 amino acid (aa) precursor protein with a putative 22 aa signal peptide that is cleaved to produce the 105 aa residue mature protein. Mature human and mouse CCL28 share 83% aa sequence identity. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Human and mouse CCL28 RNA expression is highest in epithelial cells of normal and pathologic colon. Human CCL28 RNA is also present in normal and asthmatic lung tissues. CCR10 (GPR2 orphan receptor) is the receptor for both CCL27/CTACK and CCL28.
Wang, W. et al. (2000) J. Biol. Chem. 275:22313.
Entrez Gene IDs:
56477 (Human); 56838 (Mouse)
CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.