Human CCL28 Antibody

Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody.

Recombinant Human CCL28 (Catalog # 717-VC) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL28 (4 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL28 Monoclonal Antibody (Catalog # MAB717). The ND₅₀ is typically 15-45 µg/mL.

Detection of CCL28 by Western Blot

Cervical lymphatic inflammation through VEGFR3 tyrosine kinase in mice after stroke: a Male C57BL6 mice were subjected to transient focal ischemia and, immediately after reperfusion the vehicle (PBS 10 µL) or MAZ51 (3 ng/10 µL) were injected into the nasal cavity. b MAZ51 treatment suppressed tyrosine phosphorylation in superficial CLN lymphatic endothelium at 72 h after focal ischemia (Sham; n = 4, MCAO; n = 9, MCAO + MAZ51; n = 8 biologically independent animals). *P < 0.05, one-way ANOVA followed by Fisher’s LSD test. c FACS analysis demonstrated that MAZ51 treatment significantly decreased pro-inflammatory TNF-alpha positive macrophages in superficial CLNs; no clear changes were noticed in TGF-beta positive macrophages. *P < 0.05, one-way ANOVA followed by Fisher’s LSD test. d–f. Immunostaining revealed that CD169 positive macrophages increased TNF-alpha, while MAZ51 treatment decreased pro-inflammatory macrophages. Scale: 100 nm. g–h Confocal microscopy analysis demonstrated that TNF-alpha was highly co-localized with CD169 positive macrophages in the subcapsular sinus of superficial CLNs. MAZ51 treatment decreased the co-localization. TNF-alpha expression was not observed in ILN macrophages. Scale: 100 nm. i–l Western blot confirmed that TNF-alpha, IL-1 beta and CCL28 were significantly increased in post-stroke superficial CLNs. MAZ51 treatment reduced cytokine/chemokine expression (n = 4 biologically independent animals). ILNs weakly responded to focal cerebral ischemia as to TNF-alpha, IL-1 beta or CCL28 expression (n = 3 biologically independent animals). *P < 0.05, **P < 0.01, one-way ANOVA followed by Fisher’s LSD test. All values are mean +/− SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31757960), licensed under a CC-BY license. Not internally tested by R&D Systems.