Human CCL28 Antibody

Catalog # Availability Size / Price Qty
MAB717
MAB717-SP
Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody.
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Product Details
Citations (5)
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Human CCL28 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL28 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9, 11, 19, 21, 22, 25, or recombinant rat CCL20 is observed.
Source
Monoclonal Mouse IgG1 Clone # 62705
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CCL28
Ile23-Tyr127
Accession # Q9NRJ3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL28 (Catalog # 717-VC)
Neutralization
Measured by its ability to neutralize CCL28-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR10. The Neutralization Dose (ND50) is typically 15-45 µg/mL in the presence of 4 µg/mL Recombinant Human CCL28.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody. View Larger

Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody. Recombinant Human CCL28 (Catalog # 717-VC) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL28 (4 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL28 Monoclonal Antibody (Catalog # MAB717). The ND50 is typically 15-45 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL28

Human CCL28 (CC chemokine ligand 28) is a 127 amino acid (aa) precursor protein with a putative 22 aa signal peptide that is cleaved to produce the 105 aa residue mature protein. Mature human and mouse CCL28  share 83% aa sequence identity. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Human and mouse CCL28 RNA expression is highest in epithelial cells of normal and pathologic colon. Human CCL28 RNA is also present in normal and asthmatic lung tissues. CCR10 (GPR2 orphan receptor) is the receptor for both CCL27/CTACK and  CCL28.

References
  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.
Entrez Gene IDs
56477 (Human); 56838 (Mouse)
Alternate Names
CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC

Product Datasheets

Citations for Human CCL28 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals
    Authors: M Nayrac, M Requena, C Loiseau, M Cazabat, B Suc, N Carrere, K Barange, L Alric, G Martin-Blo, J Izopet, P Delobel
    Mucosal Immunol, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  2. Brain-to-cervical lymph node signaling after stroke
    Authors: E Esposito, BJ Ahn, J Shi, Y Nakamura, JH Park, ET Mandeville, Z Yu, SJ Chan, R Desai, A Hayakawa, X Ji, EH Lo, K Hayakawa
    Nat Commun, 2019;10(1):5306.
    Species: Mouse
    Sample Types: CSF
    Applications: Western Blot
  3. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
    Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
    Nature, 2011;475(7355):226-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Epithelial inflammation is associated with CCL28 production and the recruitment of regulatory T cells expressing CCR10.
    Authors: Eksteen B, Miles A, Curbishley SM, Tselepis C, Grant AJ, Walker LS, Adams DH
    J. Immunol., 2006;177(1):593-603.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.
    Authors: Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O
    J. Immunol., 2003;170(3):1452-61.
    Species: Human
    Sample Types: Milk
    Applications: ELISA Development

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