Human CCL5/RANTES Antibody (16411)

R&D Systems | Catalog # MAB2781

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry-Paraffin, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 16411
View all CCL5/RANTES Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL5/RANTES

Specificity

Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human CCL1, 2, 3, 4, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, recombinant cotton rat CCL3, 4, 5, or recombinant rat CCL20.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL5/RANTES Antibody (16411)

Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody.

Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody.

Recombinant Human CCL5/RANTES (Catalog # 278-RN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB2781). The ND50 is typically 0.2-0.8 µg/mL.

Applications for Human CCL5/RANTES Antibody (16411)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CCL5/RANTES (Catalog # 278-RN)

Neutralization

Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL5/RANTES.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL5/RANTES

RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily and is now designated CCL5. It binds and activates the chemokine receptors CCR1, CCR3, and CCR5.

Alternate Names

RANTES, SISd

Entrez Gene IDs

6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)

Gene Symbol

CCL5

Additional CCL5/RANTES Products

Product Documents for Human CCL5/RANTES Antibody (16411)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL5/RANTES Antibody (16411)

For research use only

Citations for Human CCL5/RANTES Antibody (16411)

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