Human CD177 Antibody Summary
Accession # AAH29167
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD177 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Goat Anti-Human CD177 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3505, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD177 is a polymorphic gene in the leukocyte antigen (Ly6) uPAR superfamily. It encodes at least two alleles of the neutrophilic membrane protein PRV1 (Polycythemic Rubra Vera protein 1) and NB1 (also named HNA-2a) that are glycosyl-phosphotidylinositol (GP1) anchored proteins of 58‑64 kDa. CD177 has two highly homologous cysteine-rich uPAR/Ly6 domains. The mRNA levels of CD177 is elevated under conditions where neutrophil counts are increased and is a useful marker for polycythemic vera. Mature human and mouse CD177 share 43% amino acid sequence identity. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (1-3).
- Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
- Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
- Temerinac, S. et al. (2000) Blood 95:2569.
Citation for Human CD177 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Complement Receptor Mac-1 Is an Adaptor for NB1 (CD177)-mediated PR3-ANCA Neutrophil Activation.
Authors: Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, Kettritz R
J. Biol. Chem., 2011;286(9):7070-81.
Sample Types: Cell Lysates
Applications: Western Blot
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