Human CD25/IL-2R alpha Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry.
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Product Details

Human CD25/IL-2R alpha Antibody Summary

Species Reactivity
Detects Human CD25/IL-2R alpha in direct Elisas
Recombinant Monoclonal Rabbit IgG Clone # 2604A
Protein A or G purified from cell culture supernatant
NSO-derived recombinant Human CD25/ILR2a

Accession # P01589
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Scientific Data

Flow Cytometry View Larger

Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry. Human PBMCs were treated with Recombinant Human IL-2 (202-IL,20 ng/mL) and TGF-beta (100-B,10 ng/mL) for 24 hours, then stained with (A) Rabbit anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB10203) or (B) Rabbit IgG control antibody (MAB1050), followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (F0110) and Mouse anti-Human CD3 APC-conjugated Monoclonal Antiobdy (FAB100A). View our protocol for Staining Membrane-associated Proteins.

Blockade of Receptor-ligand Interaction View Larger

IL-2 binding to Human CD25/IL-2R alpha-transfected HEK293 Human Cell Line is Blocked by Human CD25/IL-2R alpha Antibody. In a functional flow cytometry test, Recombinant Human IL-2 Biotinylated protein (BT202, 100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha. (A) Binding is completely blocked by 2.5 µg/mL of Human Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB10203) but not by (B) Rabbit IgG Isotype Control (MAB1050) at the same concentration. Ligand binding was detected by staining cells with APC-conjugated Streptavidin (F0050).

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

Human IL-2 receptor alpha (IL-2R alpha ), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL-2. The IL-2R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2R alpha binds IL-2 with low affinity. However, when IL-2R alpha is associated with the IL-2R beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL 2 signals is formed.

Long Name
Interleukin 2 Receptor alpha
Entrez Gene IDs
3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names
CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

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