Human CD25/IL-2R alpha Antibody

Name: Human CD25/IL-2R alpha Antibody
Brand: R&D Systems
SKU: MAB10203
Price: 149 USD
Availability: InStock

R&D Systems | Catalog # MAB10203

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Product Documents

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2604A

View all CD25/IL-2R alpha Primary Antibodies »

Product Specifications

Immunogen

NSO-derived recombinant Human CD25/ILR2a

Glu22-Cys213
Accession # P01589

Specificity

Detects Human CD25/IL-2R alpha in direct Elisas.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human CD25/IL-2R alpha Antibody

Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry.

Human PBMCs were treated with Recombinant Human IL-2 (202-IL,20 ng/mL) and TGF-beta (100-B,10 ng/mL) for 24 hours, then stained with (A) Rabbit anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB10203) or (B) Rabbit IgG control antibody (MAB1050), followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (F0110) and Mouse anti-Human CD3 APC-conjugated Monoclonal Antiobdy (FAB100A). View our protocol for Staining Membrane-associated Proteins.

IL-2 binding to Human CD25/IL-2R alpha-transfected HEK293 Human Cell Line is Blocked by Human CD25/IL-2R alpha Antibody.

In a functional flow cytometry test, Recombinant Human IL-2 Biotinylated protein (BT202, 100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha. (A) Binding is completely blocked by 2.5 µg/mL of Human Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB10203) but not by (B) Rabbit IgG Isotype Control (MAB1050) at the same concentration. Ligand binding was detected by staining cells with APC-conjugated Streptavidin (F0050).

Applications for Human CD25/IL-2R alpha Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB11225). This product is intended for assay development on various assay platforms requiring antibody pairs.

Flow Cytometry

0.25 µg/mL
Sample: Human PBMCs treated with Recombinant Human IL-2 (Catalog # 202-IL,20 ng/mL) and TGF-beta (Catalog # 100-B,10 ng/mL) for 24 hours,Catalog # BT202

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD25/IL-2R alpha

Human IL-2 receptor alpha (IL-2R alpha ), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL-2. The IL-2R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2R alpha binds IL-2 with low affinity. However, when IL-2R alpha is associated with the IL-2R beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL 2 signals is formed.