Human CD25/IL-2R alpha Alexa Fluor® 700-conjugated Antibody Summary
Accession # P01589
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with plate bound Human CD3 Monoclonal Antibody (Catalog # MAB100, 10 µg/ml), Human CD28 Monoclonal Antibody (Catalog # MAB342, 5 µg/ml) and 10 ng/mL Recombinant Human TGF- beta 1 (Catalog # 240-B) for 2 days were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CD25/IL-2 R alpha Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1020N) or (B) Mouse IgG2A Alexa Fluor 700 Isotype Control (Catalog # IC003N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD25/IL-2R alpha
IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.
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