Human CD300f/LMIR3 Antibody
Human CD300f/LMIR3 Antibody Summary
Accession # Q8TDQ1
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD300f, also known as CD300LF, LMIR3, IREM-1, CLM-1, IgSF13, DIgR1, and MAIR-V, is a 50‑60 kDa glycoprotein member of the immunoglobulin superfamily (1). Human CD300f consists of a 137 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 113 aa cytoplasmic domain that contains multiple immunoreceptor tyrosine-based inhibitory motifs (ITIMs) or ITIM-like sequences (2, 3). Alternate splicing generates additional isoforms that carry substituted C-terminal tails of varying lengths and sequences following the ECD or transmembrane segment (3). Within the ECD, human CD300f shares 43% aa sequence identity with mouse and rat CD300f. CD300f is expressed on the surface of dendritic cells, monocytes, granulocytes, and mast cells as well as on acute myeloid leukemia (AML) blasts (2‑4). Pervanadate treatment or antibody crosslinking of CD300f induces phosphorylation of tyrosine residues in the cytoplasmic domain and the subsequent recruitment of phosphatases SHIP, SHP-1, SHP-2, and the p85 alpha subunit of PI3K (2, 3, 5, 6). CD300f ligation can induce cell death and inhibit signaling through multiple receptors including Fc epsilon RI, LMIR4, SCF R, TLR2, TLR3, and TLR9 (3‑8). In contrast, it enhances TLR4-mediated signaling/cytokine production in mast cells through association with the activating signaling protein FcR gamma (5). In mouse, a splice variant of CD300f (known as DIgR2, with a 7 aa insertion in the ECD) inhibits CD4+ T cell activation and in vivo Th1 and CTL responses (9). CD300f is up‑regulated on monocytes surrounding experimentally-induced spinal cord demyelination and functions as a negative regulator of inflammation in the CNS (10).
- Clark, G.J. et al. (2009) Trends Immunol. 30:209.
- Sui, L. et al. (2004) Biochem. Biophys. Res. Commun. 319:920.
- Alvarez-Errico, D. et al. (2004) Eur. J. Immunol. 34:3690.
- Korver, W. et al. (2009) Leukemia 23:1587.
- Izawa, K. et al. (2009) J. Immunol. 183:925.
- Alvarez-Errico, D. et al. (2007) J. Immunol. 178:808.
- Can, I. et al. (2008) J. Immunol. 180:207.
- Izawa, K. et al. (2007) J. Biol. Chem. 282:17997.
- Shi, L. et al. (2006) Blood 108:2678.
- Xi, H. et al. (2010) J. Exp. Med. 207:7.
Citations for Human CD300f/LMIR3 Antibody
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Citations: Showing 1 - 3
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CD300lf is the primary physiologic receptor of murine norovirus but not human norovirus
Authors: VR Graziano, FC Walker, EA Kennedy, J Wei, K Ettayebi, MS Strine, RB Filler, E Hassan, LL Hsieh, AS Kim, AO Kolawole, CE Wobus, LC Lindesmith, RS Baric, MK Estes, RC Orchard, MT Baldridge, CB Wilen
PLoS Pathog., 2020;16(4):e1008242.
Sample Types: Whole Cells
Applications: Cell Culture
Sphingomyelin and ceramide are physiological ligands for human LMIR3/CD300f, inhibiting FcepsilonRI-mediated mast cell activation.
Authors: Izawa K, Isobe M, Matsukawa T, Ito S, Maehara A, Takahashi M, Yamanishi Y, Kaitani A, Oki T, Okumura K, Kitamura T, Kitaura J
J Allergy Clin Immunol, 2014;133(1):270-3.e1-7.
Sample Types: Whole Cells
Applications: Flow Cytometry
The SRC family tyrosine kinase HCK and the ETS family transcription factors SPIB and EHF regulate transcytosis across a human follicle-associated epithelium model.
Authors: Asai, Tsuneaki, Morrison, Sherie L
J Biol Chem, 2013;288(15):10395-405.
Sample Types: Cell Lysates
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