Detection of Human CEBP epsilon by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human CEBP epsilon Monoclonal Antibody (Catalog # MAB6726) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CEBP epsilon at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
CEBP epsilon in HeLa Human Cell Line. CEBP epsilon was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human CEBP epsilon Monoclonal Antibody (Catalog # MAB6726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CEBP epsilon
CCAAT-enhancer binding protein epsilon (CEBP epsilon ) is a 32 kDa basic-leucine zipper transcription factor that is expressed in myeloblasts, granulocytes, and eosinophils. Multiple isoforms of human CEBP epsilon are generated by alternate splicing and alternative translation initiation sites. All four isoforms contain the bZIP domain (aa 208-267) but exert different effects on myeloid differentiation. Full length CEBP epsilon and the 30 kDa isoform (aa 33-281) cooperate with c-Myb to activate the transcription of genes involved in myeloid differentiation. The 27 kDa isoform (which lacks aa 69-97) and the 14 kDa isoform (which begins at Met153) function as transcriptional repressors in eosinophils. Within aa 151-210, human CEBP epsilon shares 93% amino acid sequence identity with mouse and rat CEBP epsilon, respectively.
CCAAT/enhancer-Binding Protein epsilon
Entrez Gene IDs:
1053 (Human); 110794 (Mouse); 25410 (Rat)
C/EBP-epsilon; CCAAT/enhancer binding protein (C/EBP), epsilon; CCAAT/enhancer-binding protein epsilon; CEBP epsilon; CEBPE; CRP1C/EBP epsilon
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