Human Cerberus 1 Antibody

Catalog #: AF1075 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1075
AF1075-SP

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Detection of Human Cerberus 1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cerberus 1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cerberus 1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human DAN, recombinant mouse Gremlin, and recombinant chicken Caronte is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cerberus 1
Thy18-Ala267
Accession # O95813

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Complete Your Research

Cerberus 1 antibody in Human Liver by Immunohistochemistry (IHC-P).

Human Cerberus 1 Antibody

Cat # MAB1075

Mouse Cerberus 1 Antibody

Cat # MAB1986

(1)

Citations (3)

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Cat # AF746

(1)

Citations (2)

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Cat # AF1096

(1)

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Mouse Cerberus 1 Biotinylated Antibody

Cat # BAF1986

Human Cerberus 1 Biotinylated Antibody

Cat # BAF1075

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Cat # AF5854

Dkk‑1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Mouse Dkk-1 Antibody

Cat # AF1765

(1)

Citations (7)

Stella/Dppa3 antibody in mouse ovary tissue by Immunohistochemistry (IHC-Fr).

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Cat # AF2566

Citations (1)

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sFRP-1 antibody in Human Kidney by Immunohistochemistry (IHC-P).

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Cat # AF1384

(2)

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Cat # BAF198

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Cat # MAB2256

Citations (2)

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Cat # MAB2064

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Frizzled-7 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

Human/Mouse Frizzled-7 Antibody

Cat # MAB1981

(3)

Citations (4)

Mouse Frizzled-7 Antibody

Cat # AF198

Citations (5)

Detection of VE-Cadherin antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Mouse VE-Cadherin Antibody

Cat # MAB1002

(1)

Citations (4)

PITX2 antibody in Human Thyroid Cancer Tissue by Immunohistochemistry (IHC-P).

Human PITX2 Antibody

Cat # AF7388

(2)

Citations (4)

Human Pentraxin 2/SAP Antibody

Cat # MAB1948

Citations (1)

Complete Your Experiment

Detection of Normal Goat IgG Isotype Control by Flow Cytometry

Normal Goat IgG Control

Cat # AB-108-C

(18)

Citations (335)

Detection of Goat IgG Primary Antibody by Western Blot

Goat IgG HRP-conjugated Antibody

Cat # HAF017

(8)

Citations (51)

Goat IgG HRP-conjugated Antibody

Cat # HAF109

(3)

Citations (47)

Reconstitution Buffer 1 (PBS)

Cat # RB01

(3)

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Cat # NL001

(2)

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Cat # HAF019

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Detection of Goat IgG Primary Antibody by Flow Cytometry

Goat IgG (H+L) PE-conjugated Antibody

Cat # F0107

(1)

Citations (5)

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Cat # F0108

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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Cat # NL003

Citations (8)

Detection of HAI-1 in Human Lung Cancer.

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Cat # VC004

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Cat # NL002

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Citations (7)

Goat IgG (H+L) Fluorescein-conjugated Antibody

Cat # F0109

Citations (3)

Donkey Anti-Goat IgG Biotinylated Antibody

Cat # BAF109

(1)

Citations (3)

Rabbit Anti-Goat IgG Biotinylated Antibody

Cat # BAF017

(1)

Citations (2)

Rabbit Anti-Goat IgG (H+L) Antibody, X Absorbed

Cat # R-401-C-ABS

Chicken Anti-Goat IgG Biotinylated Antibody

Cat # BAF019

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Cat # F0124

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot

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Detection of Human Cerberus 1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cerberus 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cerberus 1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cerberus 1

Cerberus 1 functions during development by binding to and antagonizing some members of the bone morphogenetic protein (BMP) family. Cerberus 1 is alternatively known as cerberus-related 1 and is a homolog of Xenopus cerberus.

Entrez Gene IDs

9350 (Human); 12622 (Mouse)

Alternate Names

CER1; cerberus 1 (Xenopus laevis) homolog (cysteine knot superfamily); Cerberus 1; cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis); cerberus; cerberus-related 1; Cerberus-related protein; DAN domain family member 4; DAND4; MGC119894; MGC119895; MGC96951