Detects human Cerberus 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DAN, recombinant mouse Gremlin, and recombinant chicken Caronte is observed.
Polyclonal Goat IgG
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cerberus 1 Thy18-Ala267 Accession # O95813
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Cerberus 1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cerberus 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cerberus 1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cerberus 1
Cerberus 1 functions during development by binding to and antagonizing some members of the bone morphogenetic protein (BMP) family. Cerberus 1 is alternatively known as cerberus-related 1 and is a homolog of Xenopus cerberus.
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