Human CHL-1/L1CAM-2 Antibody
Human CHL-1/L1CAM-2 Antibody Summary
Ile25-Gln1096
Accession # NP_006605
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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CHL‑1/L1CAM‑2 in Human Melanoma Tissue. CHL‑1/L1CAM‑2 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Rat Anti-Human CHL‑1/L1CAM‑2 Monoclonal Antibody (Catalog # MAB2126) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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CHL‑1/L1CAM‑2 in Human Spleen. CHL‑1/L1CAM‑2 was detected in immersion fixed paraffin-embedded sections of human spleen using Rat Anti-Human CHL‑1/L1CAM‑2 Monoclonal Antibody (Catalog # MAB2126) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CHL-1/L1CAM-2
Close homolog of L1 (CHL-1), also known as cell adhesion L1-like (CALL) and L1 cell adhesion molecule 2 (L1-CAM2), belongs to the L1 subfamily of the Ig superfamily cell adhesion molecules, which also include L1, neurofascin and NgCAM-related cell adhesion molecule (NrCAM) (1‑3). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4‑5 fibronectin type III-like (FNIII) domains in their extracellular regions. They also shared a highly conserved cytoplasmic region of approximately 110 amino acids (aa) containing an ankyrin-binding site. CHL-1 is expressed as a highly glycosylated 185 kDa transmembrane protein by subpopulations of neurons and glia of the central and peripheral nervous system (4, 5). Ectodomain shedding via the metalloprotease-disintegrin ADAM8 releases 165 kDa and 125 kDa soluble CHL-1 fragments, which can diffuse away to function at distant sites (6). CHL-1 is not capable of homotypic interactions, but an extracellular binding partner of CHL-1 has not been identified (4). Human CHL1 has been mapped to chromosome 3p26 and is a candidate gene for 3p- syndrome characterized by mental impairment (7). A missense CHL1 polymorphism associated with an increased risk of schizophrenia has been reported (8). The functional importance of CHL-1 in the nervous system is also evident in CHL-1 deficient mice, which display behavioral abnormalities and show misguided axons within the hippocampus and olfactory tract (9). Enhanced ectodomain-shedding of CHL-1 is also observed in Wobbler mice, the neurodegenerative mutant mice (6). In vitro, soluble or substrate-coated CHL-1 promotes neurite outgrowth and neuronal survival of both cerebellar and hippocampal neurons. Cell surface CHL-1 interacts with integrins in cis to potentiate integrin-dependent cell migration toward extracellular matrix proteins (10). For this enhanced cell motility, CHL-1 linkage to the actin cytoskeleton via interaction between ankyrin and the CHL-1 cytoplasmic region is required.
- Moos, M. et al. (1988) Nature 334:701.
- Holm, J. et al. (1996) Eur. J. Neusci. 8:1613.
- Wei, M. et al. (1998) Hum. Genet. 103:355.
- Hillenbrand, R. et al. (1999) Eur. J. Neurosci. 11:813.
- Liu, Q. et al. (2000) J. Neurosci. 20:7682.
- Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
- Angeloni, D. et al. (1999) Am. J. Med. Genet. 86:482.
- Sakurai, K. et al. (2002) Mol. Psychiatry 7:412.
- Montag-Sallaz, M. et al. (2002) Mol. Cell. Biol. 22(22):7967.
- Buhusi, M. et al. (2003) J. Biol. Chem. 278(27):25024.
Product Datasheets
Citations for Human CHL-1/L1CAM-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Expression and serum levels of the neural cell adhesion molecule L1-like protein (CHL1) in gastrointestinal stroma tumors (GIST) and its prognostic power
Authors: KF Karstens, E Bellon, A Polonski, G Wolters-Ei, N Melling, M Reeh, JR Izbicki, M Tachezy
Oncotarget, 2020;11(13):1131-1140.
Species: Human
Sample Types: Serum
Applications: ELISA Capture -
Neuregulin 1 enhances cell adhesion molecule L1 like expression levels and promotes malignancy in human glioma
Authors: WW Lin, GY Ou, JZ Lin, SJ Yi, WC Yao, HC Pan, WJ Zhao
Oncol Lett, 2020;20(1):326-336.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
BACE1 elevation engendered by GGA3 deletion increases ?-amyloid pathology in association with APP elevation and decreased CHL1 processing in 5XFAD mice
Authors: W Kim, L Ma, S Lomoio, R Willen, S Lombardo, J Dong, PG Haydon, G Tesco
Mol Neurodegener, 2018;13(1):6.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma.
Authors: Wolterink S, Moldenhauer G, Fogel M, Kiefel H, Pfeifer M, Luttgau S, Gouveia R, Costa J, Endell J, Moebius U, Altevogt P
Cancer Res., 2010;70(6):2504-15.
Species: Human
Sample Types: Recombinant Protein
Applications: Blocking
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