Human CILP-1 N-Terminal Fragment Antibody

Catalog # Availability Size / Price Qty
AF5504
AF5504-SP
Human CILP-1 N-Terminal Fragment Antibody in Western Blot
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Product Details
Citations (1)
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Human CILP-1 N-Terminal Fragment Antibody Summary

Species Reactivity
Human
Specificity
Detects human CILP‑1 N-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CILP-1 C-terminal peptide is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CILP‑1 N-Terminal Fragment
Arg22-Arg720
Accession # NP_003604
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human CILP‑1 N-Terminal Fragment by Western Blot. View Larger

Detection of Human CILP‑1 N-Terminal Fragment by Western Blot.

Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CILP‑1 N‑Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5504) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CILP‑1 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western Detection of Human CILP‑1 by Simple WesternTM. View Larger

Detection of Human CILP‑1 by Simple WesternTM. Simple Western lane view shows lysates of human cartilage tissue, loaded at 0.2 mg/mL. A specific band was detected for CILP‑1 at approximately 133 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CILP‑1 N-Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5504) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
66-440 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CILP-1

The CILP-1 (cartilage intermediate-layer protein 1) gene product is a 132 kDa (predicted) monomeric glycoprotein that is found in both hyaline and fibrocartilage. It is a precursor for two secreted, proteolytically generated products, a 90 kDa N-terminal CILP-1, and a 62 kDa C-terminal NTPPHase-homolog. The N-terminus is suggested to serve as both a matrix structural protein, and an IGF-I/TGF-beta 1 suppressor sequestration molecule. Human CILP-1 spans aa 22‑720 of the CILP-1 precursor. It contains one TSP-1 domain (aa 149‑201), a C2‑type Ig-like region (aa 309‑395) and six potential N-glycosylation sites. Over aa 1‑720 of the CILP-1 precursor, human CILP-1 shares 89% aa identity with mouse CILP‑1, and 42% aa identity with human CILP-2.

Long Name
Cartilage Intermediate Layer Protein 1
Entrez Gene IDs
8483 (Human); 214425 (Mouse); 315761 (Rat)
Alternate Names
cartilage intermediate layer protein 1 C1; cartilage intermediate layer protein 1 C2; cartilage intermediate layer protein 1; cartilage intermediate layer protein, nucleotide pyrophosphohydrolase; Cartilage intermediate-layer protein; CILP; CILP1; CILP-1; HsT18872

Product Datasheets

Citation for Human CILP-1 N-Terminal Fragment Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cartilage intermediate layer protein 1 (CILP1): A novel mediator of cardiac extracellular matrix remodelling
    Authors: FA van Nieuwe, C Munts, RC Op't Veld, A González, J Díez, S Heymans, B Schroen, M van Bilsen
    Sci Rep, 2017;7(1):16042.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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